纤连蛋白(FN)活性蛋白

Active Fibronectin (FN)

FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein

  • 纤连蛋白(FN)活性蛋白 产品包装(模拟)
  • 纤连蛋白(FN)活性蛋白 产品包装(模拟)
  • 纤连蛋白(FN)活性蛋白 Gene sequencing
  • APA037Hu02.jpg SDS-PAGE图
  • 纤连蛋白(FN)活性蛋白 Figure. Western Blot; Sample: Recombinant Fibronectin, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Fibronectin (FN) is a high-molecular weight (~440kDa) glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectin has numerous functions. For example, it involved in cell adhesion, cell motility, opsonization, wound healing, maintenance of cell shape, and so on. Besides, Connective Tissue Growth Factor (CTGF) has been identified as an interactor of FN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FN and recombinant human CTGF. Briefly, FN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTGF-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of FN and CTGF was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA037Hu02 纤连蛋白(FN)活性蛋白 Cell culture; Activity Assays.
RPA037Hu03 纤连蛋白(FN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA037Hu01 纤连蛋白(FN)活性蛋白 Cell culture; Activity Assays.
RPA037Hu01 纤连蛋白(FN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA037Hu02 纤连蛋白(FN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA037Hu01 纤连蛋白(FN)多克隆抗体 WB; IHC; ICC; IP.
PAA037Hu04 纤连蛋白(FN)多克隆抗体 WB; IHC; ICC; IP.
PAA037Hu02 纤连蛋白(FN)多克隆抗体 WB; IHC; ICC; IP.
PAA037Hu03 纤连蛋白(FN)多克隆抗体 WB; IHC; ICC; IP.
LAA037Hu71 纤连蛋白(FN)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA037Hu22 纤连蛋白(FN)单克隆抗体 WB; IHC; ICC; IP.
SEA037Hu 纤连蛋白(FN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA037Hu 纤连蛋白(FN)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA037Hu 纤连蛋白(FN)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA037Hu01 纤连蛋白(FN)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Stem Cell Research Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly [Pubmed: 24739658]
Molecules. Identification of compounds from the water soluble extract of Cinnamomum cassia barks and their inhibitory effects against high-glucose-induced mesangial cells. [Pubmed: 24013407]
Journal of Ethnopharmacology Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy [ScienceDirect: S0378874114002414]
Investigative Ophthalmology & Visual Science Inhibition by Female Sex Hormones of Collagen Gel Contraction Mediated by Retinal Pigment Epithelial Cells [Pubmed: 24609629]
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592]
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747]
Laryngoscope. Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria [Pubmed:25545625]
Biomedical Materials In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study [Pubmed:25634132]
Biomedical Materials In vivo [PubMed: 25634132]
American Journal of Medical and Biological Research Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate onNF-κB/leptin-insulin Axisin Obese Subjects with Non-alcoholic Fatty Liver Disease [Ajmbr: 3]
Food Chem. Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528]
journal of perinatal medicine Maternal endothelial damage as a disorder shared by early preeclampsia, late preeclampsia and intrauterine growth restriction. [pubmed:27865093]
Chinese Journal of Contemporary Neurology and Neurosurgery Effect of panax notoginseng saponins on efficacy and hemorrhagic transformation of rt-PA intravenous thrombolysis in patients with acute ischemic stroke [Article_en:CJFDTotal-XDJB201611014]
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