半乳糖凝集素4(GAL4)活性蛋白
Active Galectin 4 (GAL4)
LGALS4; Lactose-Binding Lectin 4; L-36 Lactose-Binding Protein; L36LBP; Lectin,Galactoside-Binding Soluble 4
- 编号APA304Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点9.0
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1512 ¥ 3780 ¥ 7560 ¥ 22680 ¥ 56700
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Galectin-4(GAL4) is a protein that in humans is encoded by the LGALS4 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS4 is an S-type lectin that is strongly underexpressed in colorectal cancer. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL4 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL4 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL4, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL4 is 3.187μg/mL.
Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA304Hu01 | 半乳糖凝集素4(GAL4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA304Hu01 | 半乳糖凝集素4(GAL4)活性蛋白 | Cell culture; Activity Assays. |
| PAA304Hu01 | 半乳糖凝集素4(GAL4)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA304Hu71 | 半乳糖凝集素4(GAL4)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA304Hu81 | 半乳糖凝集素4(GAL4)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA304Hu22 | 半乳糖凝集素4(GAL4)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA304Hu | 半乳糖凝集素4(GAL4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA304Hu | 半乳糖凝集素4(GAL4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
