生长分化因子9(GDF9)活性蛋白

Active Growth Differentiation Factor 9 (GDF9)

  • 生长分化因子9(GDF9)活性蛋白 产品包装(模拟)
  • 生长分化因子9(GDF9)活性蛋白 产品包装(模拟)
  • APA427Hu01.jpg SDS-PAGE图
  • 生长分化因子9(GDF9)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

GDF9 (Growth/differentiation factor 9) is an oocyte derived growth factora which belongs to the transforming growth factor-beta (TGFβ) superfamily. GDF9 is required for ovarian folliculogenesis and promotes primordial follicle development. S100A8 has been identified as an interactor of GDF9 through two-hybrid assay, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GDF9 and recombinant human S100A8. Briefly, GDF9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A8-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GDF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of GDF9 and S100A8 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPA427Hu01 生长分化因子9(GDF9)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA427Hu01 生长分化因子9(GDF9)活性蛋白 Cell culture; Activity Assays.
PAA427Hu01 生长分化因子9(GDF9)多克隆抗体 WB; IHC; ICC; IP.
MAA427Hu22 生长分化因子9(GDF9)单克隆抗体 WB; IHC; ICC; IP.
SEA427Hu 生长分化因子9(GDF9)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA427Hu 生长分化因子9(GDF9)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Journal of Women's medicine Follicular fluid growth differentiation factor-9 concentration and oocyte developmental competence [Ejwm: 0637JWM]
Journal of Ovarian Research Dehydroepiandrosterone improves follicular fluid bone morphogenetic protein-16 and accumulated embryo score of infertility patients with diminished ovarian reserve undergoing in vitro fertilization: a randomized controlled trial [Pubmed:25330837]
Oncotarget Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagy [Pubmed:29423092]
Development Oocytes and hypoxanthine orchestrate the G2-M switch mechanism in ovarian granulosa cells [Pubmed: 32620578]
Reprod Sci Compromised Cumulus-Oocyte Complex Matrix Organization and Expansion in Women with PCOS [34748173]
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