血红蛋白(HB)活性蛋白

Active Hemoglobin (HB)

Hgb; Haemoglobin; Heterotetramer(αβ)2

  • 血红蛋白(HB)活性蛋白 产品包装(模拟)
  • 血红蛋白(HB)活性蛋白 产品包装(模拟)
  • APB409Ha01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Hemoglobin (HB) is a protein in red blood cells which contains iron. It is used to transport oxygen around the human body. Hemoglobin is found in the red blood cells of almost all vertebrates. Hemoglobin has peroxidase activity. In the presence of hydrogen peroxide, hemoglobin can catalyze the substrates 3,3 ', 5,5' - tetramethylbenzidine (TMB) to produce a blue compound which has a maximum absorption at 595 nm. Thus, the activity of native chinese hamster hemoglobin was measured by its ability to catalyze the substrates of TMB. The reaction was performed in PBS, pH 7.4 ( Assay Buffer), ainitiated by addition 40 μL of various concentrations of HB (diluted by Assay Buffer) to 160 µL of TMB. Incubated at 37 ℃ for 20min, then read at a wavelength of 595 nm immediately. The result was shown in figure 1, and the OD value was in a linear relationship with the concentration of HB.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Hamster (Chinese hamster，仓鼠) 应用(仅供研究使用，不用于临床诊断！)
NPB409Ha01 血红蛋白(HB)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB409Ha01 血红蛋白(HB)活性蛋白 Cell culture; Activity Assays.
PAB409Ha01 血红蛋白(HB)多克隆抗体 WB; IHC; ICC; IP.
LAB409Ha71 血红蛋白(HB)多克隆抗体(生物素标记) WB; IHC; ICC.

参考文献

杂志 参考文献
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna [Pubmed:25460046]
PLoS One. Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis [Pubmed:27027307]
Parasites & Vectors The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis. [pubmed:27561965]
Proteomics. Clinical Applications Elevated levels of protein AMBP in cerebrospinal fluid of women with preeclampsia compared to normotensive pregnant women. [pubmed:27615121]
Molecular nutrition & food research Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro [pubmed:28105758]
Frontiers in Immunology Oxidized Hemoglobin Is Antigenic and Immunogenic in Lupus [pubmed:28694810]
新規人工酸素運搬体HemoAct を用いた脳虚血再灌流障害治療開発に関する研究 [:]
Acta Neuropathologica Communications Mural cell-derived laminin-α5 plays a detrimental role in ischemic stroke [Pubmed: 30777135]
Angiogenesis Decylubiquinone suppresses breast cancer growth and metastasis by inhibiting angiogenesis via the ROS/p53/BAI1 signaling pathway [Pubmed: 32020421]
Food Bioscience Water-extractable zinc protoporphyrin IX in Parma ham predominantly exists as complexes with hemoglobin and myoglobin []
Photochem Photobiol Effects of Human Serum Albumin on the Fluorescence Intensity and Tumor Imaging Properties of IR‐780 Dye [34687567]
留言咨询