Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)
BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 90%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1814 ¥ 4536 ¥ 9072 ¥ 27216 ¥ 68040
- Figure. SDS-PAGE
- Figure. Western Blot
- 通过ISO 9001、ISO 13485质量体系认证
Figure. The binding activity of IGFBP3 with EGFR.
Insulin-like growth factor-binding protein 3, also known as IGFBP3 is one of six IGF binding proteins (IGFBP1 to IGFBP6) that have highly conserved structures and bind the insulin-like growth factors IGF-1 and IGF-2 with high affinity. Within tissues, IGFBP3 can bind IGF1 and IGF2 released by many cell types, and block their access to the IGF-1 receptor (IGF1R), which is activated by both IGFs. IGFBP3 also interacts with cell-surface proteins, affecting cell signaling from outside the cell or after internalization, and also enters the cell nucleus where it binds to nuclear hormone receptors and other ligands. Besides, Epidermal Growth Factor Receptor (EGFR) has been identified as an interactor of IGFBP3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IGFBP3 and recombinant human EGFR. Briefly, IGFBP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EGFR-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IGFBP3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IGFBP3 and EGFR was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|APA054Hu01||胰岛素样生长因子结合蛋白3(IGFBP3)活性蛋白||Cell culture; Activity Assays.|
|RPA054Hu01||胰岛素样生长因子结合蛋白3(IGFBP3)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA054Hu01||胰岛素样生长因子结合蛋白3(IGFBP3)多克隆抗体||WB; IHC; ICC; IP.|
|LAA054Hu71||胰岛素样生长因子结合蛋白3(IGFBP3)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|MAA054Hu22||胰岛素样生长因子结合蛋白3(IGFBP3)单克隆抗体||WB; IHC; ICC; IP.|
|SEA054Hu||胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA054Hu01||胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒DIY材料(酶联免疫吸附试验法)||Main materials for "Do It (ELISA Kit) Yourself".|
|Molecular Biology Reports||Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male rats [PubMed: 20204528]|
|Endocrinology||Role of IGFBP-3 in the Regulation of β-Cell Mass during Obesity: Adipose Tissue/β-Cell Cross Talk [Endo: source]|
|Nutrition and Cancer||A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers [Tandfonline: Source]|
|Dig Dis Sci||The Protective Effects of Exclusive Enteral Nutrition Formulas on Growth Factor Expression and the Proximal Tibial Epiphyseal Growth Plate in a TNBS-Induced IBD Rat Model [PubMed: 25708898]|
|Animal.||Effects of a dietary crude fibre concentrate on growth in weaned piglets. [pubmed:28318464]|