10kDa干扰素γ诱导蛋白(IP10)活性蛋白

Active Interferon Gamma Induced Protein 10kDa (IP10)

CXCL10; C7; IFI10; INP10; SCYB10; Crg2; GIP10; Mob1; Chemokine C-X-C Motif Ligand 10; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10

  • 10kDa干扰素γ诱导蛋白(IP10)活性蛋白 产品包装(模拟)
  • 10kDa干扰素γ诱导蛋白(IP10)活性蛋白 产品包装(模拟)
  • APA371Hu01.png Figure. SDS-PAGE
  • 10kDa干扰素γ诱导蛋白(IP10)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of IP10 with IGFBP7.
Interferon gamma-induced protein 10 (IP10) also known as C-X-C motif chemokine 10 (CXCL10) or small-inducible cytokine B10 is an 8.7kDa protein that in humans is encoded by the CXCL10 gene. C-X-C motif chemokine 10 is a small cytokine belonging to the CXC chemokine family. IP10 secreted by several cell types in response to IFN-γ, has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. Besides, Insulin Like Growth Factor Binding Protein 7 (IGFBP7) has been identified as an interactor of IP10, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IP10 and recombinant human IGFBP7. Briefly, VDR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IGFBP7-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IP10 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IP10 and IGFBP7 was shown in Figure 1, and this effect was in a dose dependent manner.

Figure. The chemotactic effect of IP10 on Raji cells.
Chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of IP10 on the Raji cell line. Briefly, Raji cells were seeded into the upper chambers (150μL cell suspension, 106 cells/mL in RPMI 1640 with FBS free) and IP10 (1ng/mL, 10ng/mL ,100ng/mL and 1000ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows IP10 is able to induce migration of Raji cells. The migrated Raji cells in low chamber at low magnification (×100) were shown in Figure 2. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 3. The optimum chemotaxis of IP10 occurs at 10-100ng/mL.
(A) Raji cells were seeded into the upper chambers and serum free RPMI 1640 with 10ng/mL IP10 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h;
(B) Raji cells were seeded into the upper chambers and serum free RPMI 1640 without IP10 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 2h.

Figure. The chemotactic effect of IP10 on Raji cells

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA371Hu01 10kDa干扰素γ诱导蛋白(IP10)活性蛋白 Cell culture; Activity Assays.
RPA371Hu01 10kDa干扰素γ诱导蛋白(IP10)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA371Hu01 10kDa干扰素γ诱导蛋白(IP10)多克隆抗体 WB; IHC; ICC; IP.
LAA371Hu71 10kDa干扰素γ诱导蛋白(IP10)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA371Hu81 10kDa干扰素γ诱导蛋白(IP10)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA371Hu22 10kDa干扰素γ诱导蛋白(IP10)单克隆抗体 WB; IHC; ICC; IP.
SEA371Hu 10kDa干扰素γ诱导蛋白(IP10)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
PSA371Hu01 10kDa干扰素γ诱导蛋白(IP10)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA371Hu01 10kDa干扰素γ诱导蛋白(IP10)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
20 Melatonin inhibits type 1 interferon signaling of toll-like receptor 4 via heme oxygenase-1 induction in hepatic ischemia/reperfusion. [PubMed: 22288937]
BMC Pregnancy and Childbirth Maternal circulating leukocytes display early chemotactic responsiveness during late gestation [PubMed: PMC3561147]
Rheumatology International Serum levels of selected chemokines in systemic lupus erythematosus patients [Pubmed: 22461186]
PLOS ONE The Immunosuppressant Protosappanin A Diminished Recipient T Cell Migration into Allograft via Inhibition of IP-10 in Rat Heart Transplant [Pubmed:24798458]
Front Pharmacol The G-Protein-Coupled Bile Acid Receptor Gpbar1 (TGR5) Inhibits Gastric Inflammation Through Antagonizing NF-κB Signaling Pathway [PubMed: 26696888]
Cytokine Hypoxia/ischemia promotes CXCL10 expression in cardiac microvascular endothelial cells by NFkB activation [Pubmed:26891076]
Journal of Experimental & Clinical Cancer Research LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4. [pubmed:28641579]
留言咨询