白介素1α(IL1a)活性蛋白

Active Interleukin 1 Alpha (IL1a)

IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1

  • 白介素1α(IL1a)活性蛋白 产品包装(模拟)
  • 白介素1α(IL1a)活性蛋白 产品包装(模拟)
  • 白介素1α(IL1a)活性蛋白 Gene sequencing
  • APA071Hu01.jpg SDS-PAGE图
  • 白介素1α(IL1a)活性蛋白 Figure. Western Blot; Sample: Recombinant IL1a, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

IL1α (Interleukin-1 alpha) is a member of the interleukin 1 cytokine family. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces cell apoptosis. It is reported that exposure of MCF-7 cells to certain concentration of IL1α results in inhibition of cell growth. Thus, an cell proliferation assay of MCF-7 was conducted with the addition of IL1α. MCF-7 cells were seeded overnight at a density of 5,000 cells/well, and then treated with or without various concentrations of IL1α for 72h, then cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours in at 37°C.<br/>Inhibition of MCF-7 cell proliferation after incubation with IL1α for 72h observed by inverted microscope was shown in Figure 1.

Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with various concentrations of IL1α for 72h. The mean OD value of MCF-7 assessed by CCK-8 was shown in Figure 2. It was obvious that IL1α significantly decreased cell viability of MCF-7 cells.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA071Hu02 白介素1α(IL1a)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA071Hu01 白介素1α(IL1a)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA071Hu01 白介素1α(IL1a)活性蛋白 Cell culture; Activity Assays.
PAA071Hu02 白介素1α(IL1a)多克隆抗体 WB; IHC; ICC; IP.
PAA071Hu01 白介素1α(IL1a)多克隆抗体 WB; IHC; ICC; IP.
LAA071Hu71 白介素1α(IL1a)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA071Hu81 白介素1α(IL1a)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA071Hu22 白介素1α(IL1a)单克隆抗体 WB; IHC; ICC; IP.
MAA071Hu21 白介素1α(IL1a)单克隆抗体 WB; IHC; ICC; IP.
LAA071Hu72 白介素1α(IL1a)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA071Hu 白介素1α(IL1a)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
PSA071Hu01 白介素1α(IL1a)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA071Hu01 白介素1α(IL1a)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".
IGA071Hu21 白介素1α(IL1a)人基因嵌入小鼠 动物实验,阳性对照。

参考文献

杂志 参考文献
The Veterinary Journal Quantitative assessment of hsp70, IL-1β and TNF-α in the spinal cord of dogs with E40K SOD1-associated degenerative myelopathy [Pubmed: 24662024]
Int J Clin Exp Pathol Expression of S100 family proteins in neonatal rats with sepsis and its significance [PubMed: 25973048]
Tumor Biology Hypoxia-induced secretion of IL-17 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]
Parasites & Vectors Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced highlevels of humoral responses and pro-inflammatory cytokines but confers limited protection. [pubmed:27502394]
Cell Death Dis. Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy [pubmed:28333148]
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