Active Interleukin 1 Alpha (IL1a)
IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 97%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg (保证有货 万一缺货 免费重做)
- 价格 ¥ 1920 ¥ 4800 ¥ 9600 ¥ 28800 ¥ 72000
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant IL1a, Human.
- 通过ISO 9001、ISO 13485质量体系认证
IL1α (Interleukin-1 alpha) is a member of the interleukin 1 cytokine family. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces cell apoptosis. It is reported that exposure of MCF-7 cells to certain concentration of IL1α results in inhibition of cell growth. Thus, an cell proliferation assay of MCF-7 was conducted with the addition of IL1α. MCF-7 cells were seeded overnight at a density of 5,000 cells/well, and then treated with or without various concentrations of IL1α for 72h, then cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours in at 37°C.<br/>Inhibition of MCF-7 cell proliferation after incubation with IL1α for 72h observed by inverted microscope was shown in Figure 1.
Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with various concentrations of IL1α for 72h. The mean OD value of MCF-7 assessed by CCK-8 was shown in Figure 2. It was obvious that IL1α significantly decreased cell viability of MCF-7 cells.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|RPA071Hu02||白介素1α(IL1a)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA071Hu01||白介素1α(IL1a)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA071Hu01||白介素1α(IL1a)活性蛋白||Cell culture; Activity Assays.|
|PAA071Hu02||白介素1α(IL1a)多克隆抗体||WB; IHC; ICC; IP.|
|PAA071Hu01||白介素1α(IL1a)多克隆抗体||WB; IHC; ICC; IP.|
|LAA071Hu71||白介素1α(IL1a)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|LAA071Hu81||白介素1α(IL1a)多克隆抗体(异硫氰酸荧光素标记)||WB; IHC; ICC; IF.|
|MAA071Hu22||白介素1α(IL1a)单克隆抗体||WB; IHC; ICC; IP.|
|MAA071Hu21||白介素1α(IL1a)单克隆抗体||WB; IHC; ICC; IP.|
|LAA071Hu72||白介素1α(IL1a)单克隆抗体(生物素标记)||WB; IHC; ICC.|
|SEA071Hu||白介素1α(IL1a)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PSA071Hu01||白介素1α(IL1a)抗体对||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSA071Hu01||白介素1α(IL1a)检测试剂盒DIY材料(酶联免疫吸附试验法)||Main materials for "Do It (ELISA Kit) Yourself".|
|The Veterinary Journal||Quantitative assessment of hsp70, IL-1β and TNF-α in the spinal cord of dogs with E40K SOD1-associated degenerative myelopathy [Pubmed: 24662024]|
|Int J Clin Exp Pathol||Expression of S100 family proteins in neonatal rats with sepsis and its significance [PubMed: 25973048]|
|Tumor Biology||Hypoxia-induced secretion of IL-17 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]|
|Parasites & Vectors||Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced highlevels of humoral responses and pro-inflammatory cytokines but confers limited protection. [pubmed:27502394]|
|Cell Death Dis.||Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy [pubmed:28333148]|