白介素10(IL10)活性蛋白

Active Interleukin 10 (IL10)

CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor

  • 白介素10(IL10)活性蛋白 产品包装(模拟)
  • 白介素10(IL10)活性蛋白 产品包装(模拟)
  • APA056Hu61.jpg SDS-PAGE图
  • 白介素10(IL10)活性蛋白 Figure. Western Blot; Sample:APA056Hu61; Antibody: PAA056Hu06
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4 T cell activation. Further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) mediated induction of the pro-inflammatory cytokines TNFα, IL-1β, IL-12, and IFNγ made by cells such as macrophages and Th1 T cells. Therefore, THP1 cells were cultured in 24 well plates at a concentration of 106 cells/ml and activated by LPS (1ug/mL) in the absence or presence of IL-10 (5ng/mL, 10ng/mL) for 24h and production of IL-1β was determined in the supernatants by cytokine specific ELISA.
Activation of THP1 cells with LPS (1ug/mL) resulted in high levels of production of IL-1β and strong inhibitory effects of IL-10 (5ng/mL, 10ng/mL) were observed on the production of IL-1β as shown in Table 1.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA056Hu01 白介素10(IL10)活性蛋白 Cell culture; Activity Assays.
RPA056Hu01 白介素10(IL10)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA056Hu02 白介素10(IL10)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA056Hu61 白介素10(IL10)活性蛋白 Cell culture; Activity Assays.
EPA056Hu61 白介素10(IL10)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA056Hu02 白介素10(IL10)多克隆抗体 WB; IHC; ICC; IP.
PAA056Hu01 白介素10(IL10)多克隆抗体 WB; IHC; ICC; IP.
PAA056Hu06 白介素10(IL10)多克隆抗体 WB; IHC; ICC; IP.
LAA056Hu71 白介素10(IL10)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA056Hu22 白介素10(IL10)单克隆抗体 WB; IHC; ICC; IP.
MAA056Hu21 白介素10(IL10)单克隆抗体 WB; IHC; ICC; IP.
HEA056Hu 白介素10(IL10)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA056Hu 白介素10(IL10)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA056Hu 白介素10(IL10)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA056Hu01 白介素10(IL10)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

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