Active Interleukin 15 (IL15)
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 97%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 2400 ¥ 6000 ¥ 12000 ¥ 36000 ¥ 90000
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant IL15, Human.
- 通过ISO 9001、ISO 13485质量体系认证
Interleukin 15 (IL15) is a widely expressed cytokine that is structurally and functionally related to IL2, which plays an important role in many immunological diseases. IL15 also regulates T and natural killer (NK) cell activation and proliferation. To test the effect of IL15 on cells proliferation of human T lymphocyte cells, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL15. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating
the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL15 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of IL15 was shown in Figure 2. It was obvious that IL15 significantly promoted cell proliferation of Jurkat cells. The ED50 for this effect is typically 0.7240 to 5.206ng/mL.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|APA061Hu01||白介素15(IL15)活性蛋白||Cell culture; Activity Assays.|
|RPA061Hu01||白介素15(IL15)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA061Hu01||白介素15(IL15)多克隆抗体||WB; IHC; ICC; IP.|
|LAA061Hu71||白介素15(IL15)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|MAA061Hu22||白介素15(IL15)单克隆抗体||WB; IHC; ICC; IP.|
|SEA061Hu||白介素15(IL15)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|Endocrine||Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training [Pubmed: 24006180]|
|Endocrine||Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype [Pubmed:25522723]|
|Med Sci Moni||miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice [PubMed: 26087886]|
|Exercise physiology||Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle [Dspace: Handle]|
|Biotechnology Letters||Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice [s10529-017-2342-1]|
|Journal of Controlled Release||Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy. [pubmed:28408199]|