白介素15(IL15)活性蛋白

Active Interleukin 15 (IL15)

  • 白介素15(IL15)活性蛋白 产品包装(模拟)
  • 白介素15(IL15)活性蛋白 产品包装(模拟)
  • 白介素15(IL15)活性蛋白 Gene sequencing
  • APA061Hu01.jpg SDS-PAGE图
  • 白介素15(IL15)活性蛋白 Figure. Western Blot; Sample: Recombinant IL15, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Interleukin 15 (IL15) is a widely expressed cytokine that is structurally and functionally related to IL2, which plays an important role in many immunological diseases. IL15 also regulates T and natural killer (NK) cell activation and proliferation. To test the effect of IL15 on cells proliferation of human T lymphocyte cells, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL15. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating
the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL15 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of IL15 was shown in Figure 2. It was obvious that IL15 significantly promoted cell proliferation of Jurkat cells. The ED50 for this effect is typically 0.7240 to 5.206ng/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA061Hu01 白介素15(IL15)活性蛋白 Cell culture; Activity Assays.
RPA061Hu01 白介素15(IL15)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA061Hu01 白介素15(IL15)多克隆抗体 WB; IHC; ICC; IP.
LAA061Hu71 白介素15(IL15)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA061Hu22 白介素15(IL15)单克隆抗体 WB; IHC; ICC; IP.
SEA061Hu 白介素15(IL15)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
IGA061Hu21 白介素15(IL15)人基因嵌入小鼠 动物实验,阳性对照。

参考文献

杂志 参考文献
Endocrine Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training [Pubmed: 24006180]
Endocrine Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype [Pubmed:25522723]
Med Sci Moni miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice [PubMed: 26087886]
Exercise physiology Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle [Dspace: Handle]
留言咨询