白介素28B(IL28B)活性蛋白

Active Interleukin 28B (IL28B)

IFNL3; Interferon,Lambda 3; Cytokine Zcyto22

  • 白介素28B(IL28B)活性蛋白 产品包装(模拟)
  • 白介素28B(IL28B)活性蛋白 产品包装(模拟)
  • APC028Hu01.jpg Figure. SDS-PAGE
  • 白介素28B(IL28B)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of IL28B with IL10Rb.
Interleukin 28B (IL28B) is a cytokine distantly related to type I interferons and the IL-10 family. It has function on antiviral, antitumour and immunomodulatory activities. IL28B also plays a critical role in the antiviral host defense, predominantly in the epithelial tissues and acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL28B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL28B and recombinant human IL10Rb. Briefly, IL28B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL28B and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APC028Hu01 白介素28B(IL28B)活性蛋白 Cell culture; Activity Assays.
LAC028Hu81 白介素28B(IL28B)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAC028Hu71 白介素28B(IL28B)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC028Hu22 白介素28B(IL28B)单克隆抗体 WB; IHC; ICC; IP.
HEC028Hu 白介素28B(IL28B)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEC028Hu 白介素28B(IL28B)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC028Hu 白介素28B(IL28B)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

参考文献

杂志 参考文献
Journal of Antimicrobial Chemotherapy Impact of IL28B gene polymorphisms on interferon-λ3 plasma levels during pegylated interferon-α/ribavirin therapy for chronic hepatitis C in patients coinfected with HIV [PubMed: 22294646]
Cytokine Different effects of three interferons L on Toll-like receptor-related gene expression in HepG2 cells. [Pubmed: 24041672]
Hepatology Polymorphisms in melanoma differentiation‐associated gene 5 link protein function to clearance of hepatitis C virus [Pubmed:25130193]
Cytokine. Increased concentration of interferon lambda-3, interferon beta and interleukin-10 in the cerebrospinal fluid of patients with tick-borne encephalitis [Pubmed:25461389]
J Biomed Res.  Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 … [pmc:PMC4726832]
Journal of Biomedical Research Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 patients [PMC4726832]
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