Active Interleukin 28B (IL28B)
IFNL3; Interferon,Lambda 3; Cytokine Zcyto22
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg (在研产品 敬请期待)
- 价格 ¥ 1920 ¥ 4800 ¥ 9600 ¥ 28800 ¥ 72000
- Figure. SDS-PAGE
- Figure. Western Blot
- 通过ISO 9001、ISO 13485质量体系认证
Figure. The binding activity of IL28B with IL10Rb.
Interleukin 28B (IL28B) is a cytokine distantly related to type I interferons and the IL-10 family. It has function on antiviral, antitumour and immunomodulatory activities. IL28B also plays a critical role in the antiviral host defense, predominantly in the epithelial tissues and acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL28B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL28B and recombinant human IL10Rb. Briefly, IL28B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL28B and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|APC028Hu01||白介素28B(IL28B)活性蛋白||Cell culture; Activity Assays.|
|LAC028Hu81||白介素28B(IL28B)多克隆抗体(异硫氰酸荧光素标记)||WB; IHC; ICC; IF.|
|LAC028Hu71||白介素28B(IL28B)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|MAC028Hu22||白介素28B(IL28B)单克隆抗体||WB; IHC; ICC; IP.|
|HEC028Hu||白介素28B(IL28B)检测试剂盒(酶联免疫吸附试验法,高敏型)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEC028Hu||白介素28B(IL28B)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCC028Hu||白介素28B(IL28B)检测试剂盒(化学发光免疫分析法)||Chemiluminescent immunoassay for Antigen Detection.|
|Journal of Antimicrobial Chemotherapy||Impact of IL28B gene polymorphisms on interferon-λ3 plasma levels during pegylated interferon-α/ribavirin therapy for chronic hepatitis C in patients coinfected with HIV [PubMed: 22294646]|
|Cytokine||Different effects of three interferons L on Toll-like receptor-related gene expression in HepG2 cells. [Pubmed: 24041672]|
|Hepatology||Polymorphisms in melanoma differentiation‐associated gene 5 link protein function to clearance of hepatitis C virus [Pubmed:25130193]|
|Cytokine.||Increased concentration of interferon lambda-3, interferon beta and interleukin-10 in the cerebrospinal fluid of patients with tick-borne encephalitis [Pubmed:25461389]|
|J Biomed Res.||Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 … [pmc:PMC4726832]|
|Journal of Biomedical Research||Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 patients [PMC4726832]|