基质金属蛋白酶13(MMP13)活性蛋白

Active Matrix Metalloproteinase 13 (MMP13)

CLG3; Collagenase 3

  • 基质金属蛋白酶13(MMP13)活性蛋白 产品包装(模拟)
  • 基质金属蛋白酶13(MMP13)活性蛋白 产品包装(模拟)
  • APA099Hu01.png Figure. SDS-PAGE
  • 基质金属蛋白酶13(MMP13)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Matrix Metalloproteinase 13 (MMP13) is a member of the matrix metalloproteinase (MMP) family. MMP13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis. MMP13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions. In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin. Thus we have chosed casein-zymography to measure the activity of MMP13. Briefly, various concentrations of MMP13 (10µg, 5µg, 1µg, 0.1µg, 0.01µg) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat-polyacrylamide gel (SDS-PAGE; 15% gels) containing casein (1 mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP13 would hydrolyze casein nearby, which was indicated by the white bands on the gel. In this experiment we use heat-denatured MMP13 protein as negative control, and trypsase (1ug/mL) as positive control.
Result : Casein hydrolysis by recombinant human MMP13 was shown in figure 1.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA099Hu01 基质金属蛋白酶13(MMP13)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA099Hu02 基质金属蛋白酶13(MMP13)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA099Hu02 基质金属蛋白酶13(MMP13)活性蛋白 Cell culture; Activity Assays.
APA099Hu01 基质金属蛋白酶13(MMP13)活性蛋白 Cell culture; Activity Assays.
PAA099Hu01 基质金属蛋白酶13(MMP13)多克隆抗体 WB; IHC; ICC; IP.
PAA099Hu02 基质金属蛋白酶13(MMP13)多克隆抗体 WB; IHC; ICC; IP.
LAA099Hu71 基质金属蛋白酶13(MMP13)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA099Hu22 基质金属蛋白酶13(MMP13)单克隆抗体 WB; IHC; ICC; IP.
HEA099Hu 基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA099Hu 基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA099Hu 基质金属蛋白酶13(MMP13)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

参考文献

杂志 参考文献
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression [ScienceDirect: S0925443911001323]
Journal of Physiology and Biochemistry Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats [SpringerLink: h875w73171w2147w]
FEBS Journal Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta [Wiley: source]
Arthritis & Rheumatism Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice [Wiley: source]
Gene Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors [ScienceDirect: S0378111911007347]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Arthritis Rheumatol Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression. [Pubmed: 24578232]
Arthritis & Rheumatology Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression [Onlinelibrary: art.38576]
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655]
Arthritis & Rheumatol Identification of α2-Macroglobulin as a Master Inhibitor of Cartilage-Degrading Factors That Attenuates the Progression of Posttraumatic Osteoarthritis [Pubmed:24578232]
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261]
Cellular Physiology and Biochemistry Inverse Regulation of Early and Late Chondrogenic Differentiation by Oxygen Tension Provides Cues for Stem Cell-Based Cartilage Tissue Engineering [Pubmed:25632940]
Osteoarthritis and Cartilage Depletion of gangliosides enhances cartilage degradation in mice [Sciencedirect:S1063458413010364]
Biol Res The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis [PubMed: 26578310]
PLoS One Interleukin-1 Receptor Antagonist Has a Novel Function in the Regulation of Matrix Metalloproteinase-13 Expression [PubMed: 26474296]
Cell Mol Immunol  Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica [Pubmed:27157496]
Scientific Reports Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds [pubmed:27821864]
International Journal of Contemporary Medical Research Matrix Metalloproteinase-13 (MMP-13): A Novel Tumor Marker for Diagnosis of Breast Carcinoma [uploads:7]
Scientific Reports Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption. [pubmed:27640363]
Photodermatol Photoimmunol Photomed. Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice [pubmed:27995657]
Molecular Medicine Reports Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells [pubmed:28447732]
bmc musculoskeletal disorders Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. [pubmed:28187731]
Journal of Cellular Physiology Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models [pubmed:27739057]
BMC Musculoskeletal Disorders Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture [pubmed:29149841]
Journal of Applied Animal Research Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208]
Alexandria Journal of Medicine L-Carnitine ameliorates knee lesions in mono-iodoacetate induced osteoarthritis in rats [10.1016:j.ajme.2016.03.002]
National Journal of Integrated Research in Medicine Matrix Metalloproteinase-13 Can Be Used As an Independent Diagnostic and Prognostic Marker for Breast Carcinoma. [AN=123116960]
留言咨询