Mdm2 p53结合蛋白同源物(MDM2)活性蛋白

Active Mdm2 p53 Binding Protein Homolog (MDM2)

HDMX; hdm2; Mouse Double Minute 2,Human Homolog Of p53-Binding Protein; Oncoprotein Mdm2

  • Mdm2 p53结合蛋白同源物(MDM2)活性蛋白 产品包装(模拟)
  • Mdm2 p53结合蛋白同源物(MDM2)活性蛋白 产品包装(模拟)
  • APG790Hu01.jpg SDS-PAGE图
  • Mdm2 p53结合蛋白同源物(MDM2)活性蛋白 Figure. Western Blot; Sample: Recombinant MDM2, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a cellular oncoprotein that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and as an inhibitor of p53 transcriptional activation. The human homologue of this protein is sometimes called Hdm2. The p53 tumor suppressor is the key target of MDM2. It has been identified as a p53 interacting protein that represses p53 transcriptional activity and also acts as an E3 ubiquitin ligase, targeting both itself and p53 for degradation by the proteasome. MDM2 is capable of auto-polyubiquitination, and in complex with p300, a cooperating E3 ubiquitin ligase, is capable of polyubiquitinating p53. Besides, S100 Calcium Binding Protein (S100) has been identified as an interactor of MDM2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MDM2 and recombinant human S100. Briefly, MDM2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MDM2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of MDM2 and S100 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APG790Hu01 Mdm2 p53结合蛋白同源物(MDM2)活性蛋白 Cell culture; Activity Assays.
RPG790Hu01 Mdm2 p53结合蛋白同源物(MDM2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAG790Hu01 Mdm2 p53结合蛋白同源物(MDM2)多克隆抗体 WB; IHC; ICC; IP.
MAG790Hu21 Mdm2 p53结合蛋白同源物(MDM2)单克隆抗体 WB; IHC; ICC; IP.
SEG790Hu Mdm2 p53结合蛋白同源物(MDM2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Clin Cancer Res Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402]
Clin Cancer Res Inactivation of the p53–KLF6–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402]
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