Active Microfibrillar Associated Protein 2 (MFAP2)
MAGP1; Microfibril-associated glycoprotein 1
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 2074 ¥ 5184 ¥ 10368 ¥ 31104 ¥ 77760
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant MFAP2, Human.
- 通过ISO 9001、ISO 13485质量体系认证
Microfibrillar-associated protein 2 (MFAP2) is an O-glycosylated protein which excreted to the extracellular space and the extracellular matrix. MFAP2 combine biglycan and elastin to form a ternary complex. MFAP2 plays a key role in the support and distensibility of the juxtacanalicular region of these collector channels. It also can inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Besides, Fibrillin 1 (FBN1) has been identified as an interactor of MFAP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MFAP2 and recombinant human FBN1. Briefly, MFAP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FBN1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MFAP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MFAP2 and FBN1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|RPC618Hu01||微纤丝关联蛋白2(MFAP2)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC618Hu01||微纤丝关联蛋白2(MFAP2)活性蛋白||Cell culture; Activity Assays.|
|PAC618Hu01||微纤丝关联蛋白2(MFAP2)多克隆抗体||WB; IHC; ICC; IP.|