微纤丝关联蛋白2(MFAP2)活性蛋白

Active Microfibrillar Associated Protein 2 (MFAP2)

MAGP1; Microfibril-associated glycoprotein 1

仅供体外研究使用,不用于临床诊断!警防假冒,可索取进口关税税单及报关单!
  • 微纤丝关联蛋白2(MFAP2)活性蛋白 产品包装(模拟)
  • 微纤丝关联蛋白2(MFAP2)活性蛋白 产品包装(模拟)
  • 微纤丝关联蛋白2(MFAP2)活性蛋白 Gene sequencing
  • APC618Hu01.jpg SDS-PAGE图
  • 微纤丝关联蛋白2(MFAP2)活性蛋白 Figure. Western Blot; Sample: Recombinant MFAP2, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Microfibrillar-associated protein 2 (MFAP2) is an O-glycosylated protein which excreted to the extracellular space and the extracellular matrix. MFAP2 combine biglycan and elastin to form a ternary complex. MFAP2 plays a key role in the support and distensibility of the juxtacanalicular region of these collector channels. It also can inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Besides, Fibrillin 1 (FBN1) has been identified as an interactor of MFAP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MFAP2 and recombinant human FBN1. Briefly, MFAP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FBN1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MFAP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MFAP2 and FBN1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

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编号 适用物种:Homo sapiens (Human,人) 应用
RPC618Hu01 微纤丝关联蛋白2(MFAP2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC618Hu01 微纤丝关联蛋白2(MFAP2)活性蛋白 Cell culture; Activity Assays.
PAC618Hu01 微纤丝关联蛋白2(MFAP2)多克隆抗体 WB; IHC; ICC; IP.
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