肽-N4-N-乙酰基-β-D-葡萄糖胺基天门冬酰胺酶F(PNGaseF)活性蛋白

Active Peptide-N4-N-Acetyl-Beta-D-Glucosaminyl Asparagine Amidase F (PNGaseF)

Ngl; PNG; PNGase F; Glycopeptide N-glycosidase; N-glycanase

  • 肽-N4-N-乙酰基-β-D-葡萄糖胺基天门冬酰胺酶F(PNGaseF)活性蛋白 产品包装(模拟)
  • 肽-N4-N-乙酰基-β-D-葡萄糖胺基天门冬酰胺酶F(PNGaseF)活性蛋白 产品包装(模拟)
  • APX267Ge01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

PNGase F (Peptide-N-glycosidase F) is a kind of enzymes for the deglycosylation of glycoproteins. The enzyme releases asparagine-linked oligosaccharides from glycoproteins and glycopeptides by hydrolyzing the amide of the asparagine (Asn) side chain. Thus, the activity of recombinant PNGase F measured by deglycosylating Interferon Gamma (IFNg) under denatured conditions. Prepare 10×denaturing buffer(5% SDS,400mM DTT), dilute IFNg to 1 μg/μl by 1×denaturing buffer,then heat the solution to 100℃for 10 minutes to denature the glycoprotein. Cool to room temperature and microcentrifuge briefly. Dobule dilution of recombinant PNGase F by assay buffer(50mmol/L Tris(pH7.4),1% NP-40), add 10 μl denatured IFNg to 10 μl different concentration of recombinant PNGase F, incubate reaction mixture at 37℃ for 1 hour. Stop the reaction by heating to 100℃ for 5 minutes, assess deglycosylation by SDS-PAGE. In the 10 μl reaction system, the amount of PNGase F needed to remove more than 95% carbohydrates from 10 μg denatured IFNg in 1 hour at 37℃ was defined as a unit. In this procedure, one unit is equal to 2.5ng recombinant PNGase F . The results are shown in Figure 1.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Pan-species (General，通用) 应用(仅供研究使用，不用于临床诊断！)
RPX267Ge01 肽-N4-N-乙酰基-β-D-葡萄糖胺基天门冬酰胺酶F(PNGaseF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APX267Ge01 肽-N4-N-乙酰基-β-D-葡萄糖胺基天门冬酰胺酶F(PNGaseF)活性蛋白 Cell culture; Activity Assays.
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