Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Nectins and Nectin-like molecules (Necl) are families of cellular adhesion molecules involved in Ca2 -independent cellular adhesion. Four nectins have been identified in humans, namely nectin-1, nectin-2, nectin-3 and nectin-4. Nectins are ubiquitously expressed and have adhesive roles in a wide range of tissues such as the adherens junction of epithelia or the chemical synapse of the neuronal tissue. Besides, Poliovirus Receptor (PVR) has been identified as an interactor of PVRL3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PVRL3 and recombinant human PVR. Briefly, PVRL3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to PVR-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PVRL3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of PVRL3 and PVR was shown in Figure 1, and this effect was in a dose dependent manner.