蛋白酶K(PROK)活性蛋白
Active Proteinase K (PROK)
Endopeptidase K; Tritirachium alkaline proteinase; Protease K; Tritirachium alkaline proteinase; Tritirachium album serine proteinase
- 编号APX263Ge51
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 5% Trehalose.
- 性状冻干粉
- 纯度> 95%
- 等电点7.1
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 840 ¥ 2100 ¥ 4200 ¥ 12600 ¥ 31500
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Proteinase K is a typical member of the subtilisin family of proteinases .It exhibits broad substrate specificity and degrades many proteins in the native state, even in the presence of detergents.The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. Thus,the activity of recombinant Proteinase K measued by hydrolyzing casein.The reaction was performed in adding 200μl recombinant Proteinase K(dilued by 0.01mol/L Tris-HCL,pH 8.0) to 200μl 10g/L casein ,rapidly mixing,incubated for 5min at 55 ℃,stop the reaction with 400μl 0.4mol/L TCA.Clarify by filtration through a 0.45 m filter or by centrifugation. Read the supernatant an 275nm. The blank use 200μl 0.01mol/L Tris-HCL,pH 8.0 replace the Proteinase K. One unit is described as that amount of enzyme that liberates 1 µg of L- tyrosine within one minute at 55°C using casein as a substrate.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| EPX263Ge51 | 蛋白酶K(PROK)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APX263Ge51 | 蛋白酶K(PROK)活性蛋白 | Cell culture; Activity Assays. |
