核糖核酸酶A(RNase A)活性蛋白
Active Ribonuclease A (RNase A)
RNASE1; RNASE; Rnase-A; RNase UpI-1; RIB-1; Ribonuclease,RNase A Family,1(Pancreatic)
- 编号APA297Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 90%
- 等电点8.8
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1152 ¥ 2880 ¥ 5760 ¥ 17280 ¥ 43200
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产品包装(模拟)
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产品包装(模拟)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Ribonuclease A (RNASEA) is a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. RNASEA cleaves RNA on the 3' side of pyrimidine nucleotides. The protein acts to degrade ds-RNA over ss-RNA. The activity of recombinant human RNASEA measured by cleaving yeast RNA. One unit of the enzyme causes an increase in absorbance of 0.001 at 260 nm in 15 min when yeast RNA is hydrolyzed at 50°C and pH 5.0. Pipette 50ulof respective recombinant human RNASEA dilution into 100ul 0.1M sodium acetate buffer, pH 5.0, then add 150ul of 0.15mg/ml yeast RNA. The blank tube use 50ul ultrapure water instead of enzyme dilution. All the all tubes Incubate at 50°C for 15 minutes. Read A260 versus blank.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA297Hu01 | 核糖核酸酶A(RNase A)活性蛋白 | Cell culture; Activity Assays. |
| RPA297Hu01 | 核糖核酸酶A(RNase A)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA297Hu02 | 核糖核酸酶A(RNase A)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA297Hu01 | 核糖核酸酶A(RNase A)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA297Hu71 | 核糖核酸酶A(RNase A)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA297Hu22 | 核糖核酸酶A(RNase A)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA297Hu | 核糖核酸酶A(RNase A)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA297Hu | 核糖核酸酶A(RNase A)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| International Journal of Molecular Sciences | The Human Host Defense Ribonucleases 1, 3 and 7 Are Elevated in Patients with Sepsis after Major Surgery—A Pilot Study [Pubmed:26927088] |
| Investigative Ophthalmology & Visual Science | The Proteomic Landscape in the Vitreous of Patients With Age-Related and Diabetic Retinal Disease [Pubmed:30025106] |
| Nat Commun | Human ribonuclease 1 serves as a secretory ligand of ephrin A4 receptor and induces breast tumor initiation [33986289] |
