基质细胞衍生因子1(SDF1)活性蛋白

Active Stromal Cell Derived Factor 1 (SDF1)

CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12

  • 基质细胞衍生因子1(SDF1)活性蛋白 产品包装(模拟)
  • 基质细胞衍生因子1(SDF1)活性蛋白 产品包装(模拟)
  • APA122Hu02.jpg SDS-PAGE图
  • 基质细胞衍生因子1(SDF1)活性蛋白 Figure. Western Blot; Sample: Recombinant SDF1, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

SDF1 (stromal cell-derived factor 1), also known as C-X-C motif chemokine 12, is a chemokine protein that has chemotaxis active on T-lymphocytes and monocytes. It is thought that SDF1 stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3; thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of SDF1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and SDF1 (10ng/mL, 30ng/mL and 60ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8µm pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows SDF1 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of SDF1 occurs at 10ng/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA122Hu01 基质细胞衍生因子1(SDF1)活性蛋白 Cell culture; Activity Assays.
RPA122Hu02 基质细胞衍生因子1(SDF1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA122Hu62 基质细胞衍生因子1(SDF1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA122Hu01 基质细胞衍生因子1(SDF1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA122Hu02 基质细胞衍生因子1(SDF1)活性蛋白 Cell culture; Activity Assays.
EPA122Hu61 基质细胞衍生因子1(SDF1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA122Hu02 基质细胞衍生因子1(SDF1)多克隆抗体 WB; IHC; ICC; IP.
PAA122Hu01 基质细胞衍生因子1(SDF1)多克隆抗体 WB; IHC; ICC/IF;FCM
LAA122Hu71 基质细胞衍生因子1(SDF1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA122Hu21 基质细胞衍生因子1(SDF1)单克隆抗体 WB; IHC; ICC; IP.
MAA122Hu23 基质细胞衍生因子1(SDF1)单克隆抗体 WB; IHC; ICC; IP.
MAA122Hu22 基质细胞衍生因子1(SDF1)单克隆抗体 WB
SEA122Hu 基质细胞衍生因子1(SDF1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA122Hu 基质细胞衍生因子1(SDF1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA122Hu01 基质细胞衍生因子1(SDF1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

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