Active Tubulin Beta (TUBb)
TUB-B; TUBB5; TUBB1; M40
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1987 ¥ 4968 ¥ 9936 ¥ 29808 ¥ 74520
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant TUBb, Human.
- 通过ISO 9001、ISO 13485质量体系认证
Tubulin Beta (TUBb) in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. It participate in many essential cellular processes, including mitosis. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Both α and β tubulins have a mass of around 50kDa and are thus in a similar range compared to actin with ~42kDa. TUBb is one of six members of the tubulin superfamily, which binding drugs to kill cancerous cells by inhibiting microtubule dynamics. Besides, Myxovirus Resistance 1 (MX1) has been identified as an interactor of TUBb, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TUBb and recombinant human MX1. Briefly, TUBb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MX1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TUBb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TUBb and MX1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|APB870Hu01||微管蛋白β(TUBb)活性蛋白||Cell culture; Activity Assays.|
|RPB870Hu01||微管蛋白β(TUBb)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|CAB870Hu01||抗微管蛋白β(TUBb)多克隆抗体||Loading Control of WB.|
|PAB870Hu01||微管蛋白β(TUBb)多克隆抗体||WB; IHC; ICC; IP.|
|MAB870Hu21||微管蛋白β(TUBb)单克隆抗体||WB; IHC; ICC; IP.|
|CAB870Hu22||抗微管蛋白β(TUBb)单克隆抗体||Loading Control of WB.|
|archives of pharmacal research||Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analogues [pubmed:27747473]|
|archiv der pharmazie (Weinheim).||New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition. [pubmed:28150327]|
|ACS Applied Materials & Interfaces||Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixture [pubmed:28112513]|