微管蛋白β(TUBb)活性蛋白

Active Tubulin Beta (TUBb)

TUB-B; TUBB5; TUBB1; M40

  • 微管蛋白β(TUBb)活性蛋白 产品包装(模拟)
  • 微管蛋白β(TUBb)活性蛋白 产品包装(模拟)
  • 微管蛋白β(TUBb)活性蛋白 Gene sequencing
  • APB870Hu01.jpg Figure. SDS-PAGE
  • 微管蛋白β(TUBb)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin Beta (TUBb) is a spherical molecule which is composed of 455 amino acids. Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) has a remarkably high affinity for TUBb, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TUBb and recombinant human YWHAz. Briefly, TUBb was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to YWHAz-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TUBb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human TUBb and recombinant human YWHAz was shown in Figure 1, the EC50 for this effect is 0.88 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APB870Hu01 微管蛋白β(TUBb)活性蛋白 Cell culture; Activity Assays.
RPB870Hu01 微管蛋白β(TUBb)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CAB870Hu01 抗微管蛋白β(TUBb)多克隆抗体 Loading Control of WB.
PAB870Hu01 微管蛋白β(TUBb)多克隆抗体 WB; IHC; ICC/IF
MAB870Hu22 微管蛋白β(TUBb)单克隆抗体 WB; IHC; ICC; IP.
MAB870Hu23 微管蛋白β(TUBb)单克隆抗体 WB; IHC; ICC; IP.
MAB870Hu25 微管蛋白β(TUBb)单克隆抗体 WB; IHC; ICC; IP.
MAB870Hu27 微管蛋白β(TUBb)单克隆抗体 WB; IHC; ICC; IP.
MAB870Hu24 微管蛋白β(TUBb)单克隆抗体 WB; IHC; ICC; IP.
MAB870Hu21 微管蛋白β(TUBb)单克隆抗体 WB; IHC; ICC; IP.
CAB870Hu22 抗微管蛋白β(TUBb)单克隆抗体 Loading Control of WB.
FAB870Hu06 抗微管蛋白β(TUBb)单克隆抗体 Flow cytometry.
FAB870Hu04 抗微管蛋白β(TUBb)单克隆抗体 Flow cytometry.
FAB870Hu02 抗微管蛋白β(TUBb)单克隆抗体 Flow cytometry.
FAB870Hu08 抗微管蛋白β(TUBb)单克隆抗体 Flow cytometry.

参考文献

杂志 参考文献
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