微管蛋白β(TUBb)活性蛋白

Active Tubulin Beta (TUBb)

TUB-B; TUBB5; TUBB1; M40

  • 微管蛋白β(TUBb)活性蛋白 产品包装(模拟)
  • 微管蛋白β(TUBb)活性蛋白 产品包装(模拟)
  • 微管蛋白β(TUBb)活性蛋白 Gene sequencing
  • APB870Hu01.jpg SDS-PAGE图
  • 微管蛋白β(TUBb)活性蛋白 Figure. Western Blot; Sample: Recombinant TUBb, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Tubulin Beta (TUBb) in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. It participate in many essential cellular processes, including mitosis. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Both α and β tubulins have a mass of around 50kDa and are thus in a similar range compared to actin with ~42kDa. TUBb is one of six members of the tubulin superfamily, which binding drugs to kill cancerous cells by inhibiting microtubule dynamics. Besides, Myxovirus Resistance 1 (MX1) has been identified as an interactor of TUBb, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TUBb and recombinant human MX1. Briefly, TUBb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MX1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TUBb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TUBb and MX1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APB870Hu01 微管蛋白β(TUBb)活性蛋白 Cell culture; Activity Assays.
RPB870Hu01 微管蛋白β(TUBb)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CAB870Hu01 抗微管蛋白β(TUBb)多克隆抗体 Loading Control of WB.
PAB870Hu01 微管蛋白β(TUBb)多克隆抗体 WB; IHC; ICC; IP.
MAB870Hu21 微管蛋白β(TUBb)单克隆抗体 WB; IHC; ICC; IP.
CAB870Hu22 抗微管蛋白β(TUBb)单克隆抗体 Loading Control of WB.

参考文献

杂志 参考文献
archives of pharmacal research Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analogues [pubmed:27747473]
archiv der pharmazie (Weinheim). New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition. [pubmed:28150327]
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