血管内皮生长因子A(VEGFA)活性蛋白

Active Vascular Endothelial Growth Factor A (VEGFA)

VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor

  • 血管内皮生长因子A(VEGFA)活性蛋白 产品包装(模拟)
  • 血管内皮生长因子A(VEGFA)活性蛋白 产品包装(模拟)
  • 血管内皮生长因子A(VEGFA)活性蛋白 Figure. Gene Sequencing (Extract)
  • APA143Hu01.png Figure. SDS-PAGE
  • 血管内皮生长因子A(VEGFA)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. Cell proliferation of ECV-304 cells after stimulated with VEGFA.
Vascular endothelial growth factor A (VEGFA) is a protein that in humans is encoded by the VEGFA gene. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. VEGFA shows prominent activity with vascular endothelial cells, primarily through its interactions with the VEGFR1 and -R2 receptors found in prominently on the endothelial cell membrane. Thus, proliferation assay of recombinant human VEGFA was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640  prior to the addition of various concentrations of VEGFA. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of ECV-304 cells after incubation with VEGFA for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant VEGFA for 48h. The result was shown in Figure 2. It was obvious that VEGFA significantly increased cell viability of ECV-304 cells.
(A) ECV-304 cells cultured in 1640, stimulated with 100ng/mL VEGFA for 48h;
(B) Unstimulated ECV-304 cells cultured in 1640 for 48h.

Figure. Cell proliferation of ECV-304 cells after stimulated with VEGFA.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA143Hu02 血管内皮生长因子A(VEGFA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA143Hu01 血管内皮生长因子A(VEGFA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA143Hu61 血管内皮生长因子A(VEGFA)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA143Hu01 血管内皮生长因子A(VEGFA)活性蛋白 Cell culture; Activity Assays.
PAA143Hu01 血管内皮生长因子A(VEGFA)多克隆抗体 WB,IHC
PAA143Hu02 血管内皮生长因子A(VEGFA)多克隆抗体 WB; IHC; ICC; IP.
LAA143Hu71 血管内皮生长因子A(VEGFA)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA143Hu81 血管内皮生长因子A(VEGFA)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
RAA143Hu21 血管内皮生长因子A(VEGFA)重组抗体 WB; IF; ICC; IHC; IP; FCM.
MAA143Hu24 血管内皮生长因子A(VEGFA)单克隆抗体 WB
MAA143Hu26 血管内皮生长因子A(VEGFA)单克隆抗体 WB
MAA143Hu21 血管内皮生长因子A(VEGFA)单克隆抗体 WB
FAA143Hu02 抗血管内皮生长因子A(VEGFA)单克隆抗体 Flow cytometry.
LAA143Hu76 血管内皮生长因子A(VEGFA)单克隆抗体(生物素标记) WB; IHC; ICC.
LMA143Hu 血管内皮生长因子A(VEGFA)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
HEA143Hu 血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA143Hu 血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA143Hu 血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA143Hu 血管内皮生长因子A(VEGFA)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
PSA143Hu01 血管内皮生长因子A(VEGFA)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA143Hu01 血管内皮生长因子A(VEGFA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

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