抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody
G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH
- 编号CAB932Mi22
- 物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度1mg/ml
- 且适物种Hamster (Chinese hamster，仓鼠), Oryctolagus cuniculus (Rabbit，兔), Sus scrofa; Porcine (Pig，猪), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，绵羊), Equus caballus; Equine (Horse，马), Chicken (Gallus，鸡)
- 应用Loading Control of WB
- 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 644 ¥ 1504 ¥ 2148 ¥ 5370 ¥ 21480
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
-
DAB staining on IHC-P;
Sample: Human Rectum Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20μg/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
-
Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Heart lysate; Lane3: A549 cell lysate; Lane4: HepG2 cell lysate Primary Ab: 1µg/ml Mouse Anti-Multi-species GAPDH Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
-
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Placenta lysate; Lane3: Porcine Cerebrum lysate; Lane4: Bovine Cerebrum lysate; Lane5: Gallus Cerebrum lysate; Lane6: Equine Liver lysate; Lane7: Caprine Cerebrum lysate<br/>Primary Ab: 3µg/ml Mouse Anti-Multi-species GAPDH Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
-
Western Blot analysis of GAPDH in various cell lines
Lane1: DU145 whole cell lysate
Lane2: OS-RC-2 whole cell lysate
Lane3: T-47D whole cell lysate
Lane4: HEC-1B whole cell lysate
Lane5: HepG2 whole cell lysate
Lane6: HEK-293 whole cell lysate
Lysates/proteins at 20µg per lane.
Primary Ab: anti-GAPDH antibody (CAB932Mi22) at 0.1ng/ml
Secondary Ab: HRP-conjugated Rabbit anti-mouse antibody (SAA544Mu09) at 1/10000 dilution
Developed using the ECL technique.
Calculated MW: 36KD
Observed MW: 36KD
Exposure time: 1 min -
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Cavia Liver lysate; Lane4: Cavia Kidney lysate; Lane5: Gallus Kidney lysate; Lane6: Bovine Liver lysate; Lane7: Bovine Kidney lysate; Lane8: Rabbit Kidney lysate; Lane9: Caprine Liver lysate; Lane10: Mouse Liver lysate; Lane11: Rat Liver lysate; Lane12: Rat Kidney lysate
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
-
通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对GAPDH的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别GAPDH。
用法
Enzyme-Linked Immune Sorbent Assay:
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| CAB932Mi01 | 抗甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体 | Loading Control of WB. |
| CAB932Mi22 | 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 | Loading Control of WB |
参考文献
| 杂志 | 参考文献 |
| Clin Cancer Res | Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402] |
| Clin Cancer Res | Inactivation of the p53–KLF10–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402] |
| Diabetes & Metabolism | Dapagliflozin modulates glucagon secretion in an SGLT2-independent manner in murine alpha cells. [pubmed:28499695] |
| Scientific Reports | Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egression [pubmed:28720868] |
| Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167] |
| Biomaterials | Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels [Pubmed: 30343256] |
| Bulletin of Experimental Biology and Medicine | Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells [Pubmed: 30488202] |
| Journal of Medicinal Chemistry | Discovery, Optimization and Target Identification of Novel Potent Broad-Spectrum Antiviral Inhibitors [Pubmed: 30938999] |
| International Immunopharmacology | Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway [Pubmed: 31078923] |
| Cancers | Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia [Pubmed: 31718075] |
| Biological research | Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … [Pubmed: 32046779] |
| Biomed Pharmacother | Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway [Pubmed: 32028241] |
| Metab Brain Dis | Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in rats [Pubmed: 33170419] |
| South African Journal of Botany | Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo [] |
| Regulation of hepatic lipid metabolism in CB1-/-and Abcb4-/--HBV surface protein transgenic mice [] | |
| Cancers (Basel) | BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 581 [33540760] |
| MOLECULAR CANCER RESEARCH | Omega-3 Eicosapentaenoic Acid Reduces Prostate Tumor Vascularity [33262291] |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026] |
| Cancer Rep (Hoboken) | Curcumin alters distinct molecular pathways in breast cancer subtypes revealed by integrated miRNA/mRNA expression analysis [Pubmed:34981672] |
| Prog Neurobiol | Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegeneration [Pubmed:35151792] |
| Plant Molecular Biology | Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiency [Pubmed:35577991] |
留言咨询
