抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体

Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody

G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH

  • 编号CAB932Mi22
  • 物种Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
  • 纯化方式蛋白A + 蛋白G亲和纯化
  • 标记物无标记物
  • 缓冲液成份0.01M 磷酸盐缓冲液(pH7.4,containing 0.05% Proclin-300,50% glycerol)
  • 性状液体
  • 浓度1mg/ml
  • 且适物种Hamster (Chinese hamster,仓鼠), Oryctolagus cuniculus (Rabbit,兔), Sus scrofa; Porcine (Pig,猪), Bos taurus; Bovine (Cattle,牛), Capra hircus; Caprine (Goat,山羊), Ovis aries; Ovine (Sheep,绵羊), Equus caballus; Equine (Horse,马), Chicken (Gallus,鸡)
  • 应用Loading Control of WB
  • 下载 英文说明书   中文说明书
  • 规格 20µl100µl 200µl 1ml 10ml
  • 价格 ¥ 644 ¥ 1504 ¥ 2148 ¥ 5370 ¥ 21480
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  • 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 产品包装(模拟)
  • 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 产品包装(模拟)
  • 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 5ug/ml Rabbit Anti-Multi-species GAPDH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 DAB staining on IHC-P;
    Sample: Human Rectum Tissue;
    Primary Ab: 5ug/ml Rabbit Anti-Multi-species GAPDH Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 DAB staining on IHC-P;
    Sample: Human Liver cancer Tissue;
    Primary Ab: 5ug/ml Rabbit Anti-Multi-species GAPDH Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 DAB staining on IHC-P;
    Sample: Human Cerebrum Tissue;
    Primary Ab: 5ug/ml Rabbit Anti-Multi-species GAPDH Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 DAB staining on IHC-P;
    Sample: Human Lung Tissue;
    Primary Ab: 5ug/ml Rabbit Anti-Multi-species GAPDH Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 DAB staining on IHC-P;
    Sample: Human Ovarian cancer Tissue;
    Primary Ab: 5ug/ml Rabbit Anti-Multi-species GAPDH Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CAB932Mi22.jpg Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Heart lysate; Lane3: A549 cell lysate; Lane4: HepG2 cell lysate Primary Ab: 1µg/ml Mouse Anti-Multi-species GAPDH Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Placenta lysate; Lane3: Porcine Cerebrum lysate; Lane4: Bovine Cerebrum lysate; Lane5: Gallus Cerebrum lysate; Lane6: Equine Liver lysate; Lane7: Caprine Cerebrum lysate
    Primary Ab: 3µg/ml Mouse Anti-Multi-species GAPDH Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 Western Blot analysis of GAPDH in various cell lines
    Lane1: DU145 whole cell lysate
    Lane2: OS-RC-2 whole cell lysate
    Lane3: T-47D whole cell lysate
    Lane4: HEC-1B whole cell lysate
    Lane5: HepG2 whole cell lysate
    Lane6: HEK-293 whole cell lysate
    Lysates/proteins at 20µg per lane.
    Primary Ab: anti-GAPDH antibody (CAB932Mi22) at 0.1ng/ml
    Secondary Ab: HRP-conjugated Rabbit anti-mouse antibody (SAA544Mu09) at 1/10000 dilution
    Developed using the ECL technique.
    Calculated MW: 36KD
    Observed MW: 36KD
    Exposure time: 1 min
  • 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 Western Blot; Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Cavia Liver lysate; Lane4: Cavia Kidney lysate; Lane5: Gallus Kidney lysate; Lane6: Bovine Liver lysate; Lane7: Bovine Kidney lysate; Lane8: Rabbit Kidney lysate; Lane9: Caprine Liver lysate; Lane10: Mouse Liver lysate; Lane11: Rat Liver lysate; Lane12: Rat Kidney lysate
    Primary Ab: 0.5µg/ml Mouse Anti-Multi-species GAPDH Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对GAPDH的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别GAPDH。

用法

Western blotting: 0.0001-0.1ug/ml;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
CAB932Mi01 抗甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体 Loading Control of WB.
CAB932Mi22 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 Loading Control of WB

参考文献

杂志 参考文献
Clin Cancer Res Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402]
Clin Cancer Res Inactivation of the p53–KLF10–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402]
Diabetes & Metabolism Dapagliflozin modulates glucagon secretion in an SGLT2-independent manner in murine alpha cells. [pubmed:28499695]
Scientific Reports Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egression [pubmed:28720868]
Journal of Biological Chemistry Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167]
Biomaterials Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels [Pubmed: 30343256]
Bulletin of Experimental Biology and Medicine Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells [Pubmed: 30488202]
Journal of Medicinal Chemistry Discovery, Optimization and Target Identification of Novel Potent Broad-Spectrum Antiviral Inhibitors [Pubmed: 30938999]
International Immunopharmacology Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway [Pubmed: 31078923]
Cancers Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia [Pubmed: 31718075]
Biological research Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … [Pubmed: 32046779]
Biomed Pharmacother Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway [Pubmed: 32028241]
Metab Brain Dis Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in rats [Pubmed: 33170419]
South African Journal of Botany Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo []
Regulation of hepatic lipid metabolism in CB1-/-and Abcb4-/--HBV surface protein transgenic mice []
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