CD276分子(CD276)多克隆抗体
Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)
B7-H3; B7H3; CD276 Molecule; B7 homolog 3; Costimulatory molecule
- 编号PAA781Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPA781Hu01-CD276分子(CD276)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度0.5mg/ml
- 且适物种-
- 应用WB,IHC,FCM
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human CD276 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Vector Red staining on IHC-P;
Samples: Human Placenta Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody -
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant CD276, Human.
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Western Blot; Sample: Lane1: 293T cell lysate; Lane2: PC3 cell lysate; Lane3: MCF7 cell lysate
Primary Ab: 3µg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: CD276 knockout 293T cell lysate;
Predicted MW: 57,53,33kd
Observed MW: 100kd
Primary Ab: 3µg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
ChoK1 transfected with human CD276（Stable transfection） was stained with rabbit Anti-human CD276 Polyclonal Antibody (Catalog # PAA781Hu01), filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled blue histogram), followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb11). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对CD276的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CD276。
用法
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA781Hu01 | CD276分子(CD276)活性蛋白 | Cell culture; Activity Assays. |
| RPA781Hu01 | CD276分子(CD276)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA781Hu01 | CD276分子(CD276)多克隆抗体 | WB,IHC,FCM |
| FAA781Hu01 | 抗CD276分子(CD276)多克隆抗体 | Flow cytometry. |
| MAA781Hu22 | CD276分子(CD276)单克隆抗体 | WB |
| MAA781Hu21 | CD276分子(CD276)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA781Hu | CD276分子(CD276)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA781Hu | CD276分子(CD276)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
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