CD276分子(CD276)多克隆抗体

Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)

B7-H3; B7H3; CD276 Molecule; B7 homolog 3; Costimulatory molecule

  • CD276分子(CD276)多克隆抗体 产品包装(模拟)
  • CD276分子(CD276)多克隆抗体 产品包装(模拟)
  • CD276分子(CD276)多克隆抗体 Figure. DAB staining on IHC-P; Samples:Human Liver Cancer Tissue.
  • CD276分子(CD276)多克隆抗体 Figure. DAB staining on IHC-P; Samples:Human Prostate Tissue.
  • CD276分子(CD276)多克隆抗体 Figure. DAB staining on IHC-P; Samples:Human Kidney Tissue.
  • PAA781Hu01.jpg Figure. Western Blot; Sample: Recombinant CD276, Human.
  • CD276分子(CD276)多克隆抗体 Figure. Western Blot; Sample: Lane1: Human 293T Cells; Lane2: Human PC-3 Cells; Lane3: Human MCF-7 Cells.
  • CD276分子(CD276)多克隆抗体 Knockout Varification:
    Lane 1: Wild-type 293T cell lysate;
    Lane 2: CD276 knockout 293T cell lysate;
    Predicted MW: 57,53,33kd
    Observed MW: 100kd
    Primary Ab: 3µg/ml Rabbit Anti-Human CD276 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CD276分子(CD276)多克隆抗体 ChoK1 transfected with human CD276(Stable transfection) was stained with rabbit Anti-human CD276 Polyclonal Antibody (Catalog # PAA781Hu01), filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled blue histogram), followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb11). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对CD276的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CD276。

用法

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA781Hu01 CD276分子(CD276)活性蛋白 Cell culture; Activity Assays.
RPA781Hu01 CD276分子(CD276)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA781Hu01 CD276分子(CD276)多克隆抗体 WB; IHC; ICC; IP.
MAA781Hu22 CD276分子(CD276)单克隆抗体 WB; IHC; ICC; IP.
SEA781Hu CD276分子(CD276)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
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