谷胱甘肽过氧化酶4(GPX4)多克隆抗体
Polyclonal Antibody to Glutathione Peroxidase 4 (GPX4)
MCSP; PHGPx; SnGPx; SnPHGPx; Phospholipid Hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
- 编号PAC994Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPC994Hu01-谷胱甘肽过氧化酶4(GPX4)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度0.5mg/ml
- 且适物种Mus musculus (Mouse，小鼠)
- 应用WB,IHC
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human GPX4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Thyroid cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GPX4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GPX4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GPX4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human GPX4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Testis Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human GPX4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Stomach Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human GPX4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human GPX4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant GPX4, Human.
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Western Blot; Sample: Human Liver lysate; <br/>Primary Ab: 1µg/ml Rabbit Anti-Human GPX4 Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
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Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: GPX4 knockout 293T cell lysate;
Predicted MW: 22kd
Observed MW: 20kd
Primary Ab: 1µg/ml Rabbit Anti-Human GPX4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: 293T cell lysate; <br/>Primary Ab: 1µg/ml Rabbit Anti-Human GPX4 Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
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Western Blot; Samples: Lane1: Rat Testis lysate; Lane2: Mouse Testis lysate;
Primary Ab: 0.2µg/ml Rabbit Anti-Human GPX4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对GPX4的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别GPX4。
用法
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC994Hu01 | 谷胱甘肽过氧化酶4(GPX4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC994Hu01 | 谷胱甘肽过氧化酶4(GPX4)多克隆抗体 | WB,IHC |
| MAC994Hu21 | 谷胱甘肽过氧化酶4(GPX4)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC994Hu | 谷胱甘肽过氧化酶4(GPX4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC994Hu | 谷胱甘肽过氧化酶4(GPX4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSC994Hu01 | 谷胱甘肽过氧化酶4(GPX4)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Biomedicine & Pharmacotherapy | Artesunate alleviates liver fibrosis by regulating ferroptosis signaling pathway [Pubmed: 30551460] |
| Acta Biomater | An all-in-one biomimetic iron-small interfering RNA nanoplatform induces ferroptosis for cancer therapy [Pubmed:35709941] |
| Antioxidants | Vulnerability of Triple-Negative Breast Cancer to Saponin Formosanin C-Induced Ferroptosis [Pubmed:35204181] |
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