肿瘤蛋白p53(TP53)多克隆抗体

Polyclonal Antibody to Tumor Protein p53 (TP53)

p53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

  • 肿瘤蛋白p53(TP53)多克隆抗体 产品包装(模拟)
  • 肿瘤蛋白p53(TP53)多克隆抗体 产品包装(模拟)
  • 肿瘤蛋白p53(TP53)多克隆抗体 Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue
  • PAA928Hu01.jpg Figure 1. Western Blot; Sample: Recombinant TP53, Human.
  • 肿瘤蛋白p53(TP53)多克隆抗体 Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 肿瘤蛋白p53(TP53)多克隆抗体 Knockout Varification: <br/>Lane 1: Wild-type MCF7 cell lysate; <br/>Lane 2: TP53 knockout MCF7 cell lysate; <br/>Predicted MW: 24,30,33,34,38,39,44kDa <br/>Observed MW: 50kDa<br/>Primary Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对TP53的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别TP53。

用法

Western blotting: 0.5-2μg/mL;1:100-400
Immunohistochemistry: 5-20μg/mL;1:10-40
Immunocytochemistry: 5-20μg/mL;1:10-40
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA928Hu01 肿瘤蛋白p53(TP53)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA928Hu01 肿瘤蛋白p53(TP53)多克隆抗体 WB; IHC; ICC; IP.
LAA928Hu71 肿瘤蛋白p53(TP53)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA928Hu22 肿瘤蛋白p53(TP53)单克隆抗体 WB; IHC; ICC; IP.
SEA928Hu 肿瘤蛋白p53(TP53)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA928Hu 肿瘤蛋白p53(TP53)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA928Hu01 肿瘤蛋白p53(TP53)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Anticancer Research Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines [PubMed: 23155233]
Microscopy Research and Technique Therapeutic role of curcumin in oxidative DNA damage caused by formaldehyde [PubMed: 25761397]
J Nutr Biochem Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing mice [PubMed: 26344777]
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