核仁磷酸蛋白(NPM1)多克隆抗体
Polyclonal Antibody to Nucleophosmin 1 (NPM1)
B23; NPM; Nucleolar Phosphoprotein B23; Numatrin; Nucleophosmin/Nucleoplasmin Family,Member 1; Nucleolar protein NO38
- 编号PAC664Mu02
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPC664Mu02-核仁磷酸蛋白(NPM1)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度500µg/mL
- 且适物种Homo sapiens (Human，人)
- 应用WB,IHC,ICC/IF
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 648 ¥ 1512 ¥ 2160 ¥ 5400 ¥ 21600
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Mouse Adipose tissue Tissue; Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Stomach Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Small intestine Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Pancreas Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Uterus Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Cerebrum Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Uterus Tissue
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 Cells;
Primary Ab: 10µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 1µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: MCF7 cell
Primary Ab: 7µg/ml Rabbit Anti-Mouse NPM1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant NPM, Mouse.
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Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Jurkat cell lysate; Lane3: K562 cell lysate
Primary Ab: 1µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: NPM knockout Jurkat cell lysate;
Predicted MW: 33kd
Observed MW: 38kd
Primary Ab: 1µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对NPM1的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别NPM1。
用法
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC664Mu01 | 核仁磷酸蛋白(NPM1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC664Mu02 | 核仁磷酸蛋白(NPM1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC664Mu02 | 核仁磷酸蛋白(NPM1)多克隆抗体 | WB,IHC,ICC/IF |
| PAC664Mu01 | 核仁磷酸蛋白(NPM1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAC664Mu71 | 核仁磷酸蛋白(NPM1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
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