O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)多克隆抗体

Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)

Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase

  • O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)多克隆抗体 产品包装(模拟)
  • O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)多克隆抗体 产品包装(模拟)
  • PAC027Hu01.jpg WB图
  • O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)多克隆抗体 Figure. Western Blot; Sample: Human Jurkat Cells.
  • O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)多克隆抗体 Knockout Varification:
    Lane 1: Wild-type Jurkat cell lysate;
    Lane 2: MGMT knockout Jurkat cell lysate;
    Predicted MW: 21kd
    Observed MW: 22kd
    Primary Ab: 3µg/ml Rabbit Anti-Human MGMT Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对MGMT的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别MGMT。

用法

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPC027Hu01 O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC027Hu01 O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)多克隆抗体 WB; IHC; ICC; IP.
MAC027Hu22 O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)单克隆抗体 WB; IHC; ICC; IP.
SEC027Hu O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Biotechnology and Bioengineering Targeted nitric oxide delivery preferentially induces glioma cell chemosensitivity via altered p53 and O(6) -Methylguanine-DNA Methyltransferase activity [PubMed: 23125026]
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