结缔组织生长因子(CTGF)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Connective Tissue Growth Factor (CTGF)

CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2

  • 结缔组织生长因子(CTGF)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 结缔组织生长因子(CTGF)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 结缔组织生长因子(CTGF)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA010Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测结缔组织生长因子(CTGF),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的结缔组织生长因子(CTGF)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 92-101 97
EDTA plasma(n=5) 78-93 87
heparin plasma(n=5) 93-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的结缔组织生长因子(CTGF),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中结缔组织生长因子(CTGF)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 83-104% 78-88% 80-97%
EDTA plasma(n=5) 85-97% 96-103% 84-102% 81-92%
heparin plasma(n=5) 89-96% 81-96% 94-102% 80-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

试剂盒内容

试剂名称 数量 试剂名称 数量
96孔板(预包被) 1 96孔板覆膜 4
标准品 2 标准品稀释液 1×20mL
检测溶液A 1×120µL 检测稀释液A 1×12mL
检测溶液B 1×120µL 检测稀释液B 1×12mL
底物A 1×10mL 底物B 1×2mL
浓洗涤液 (30 × concentrate) 1×20mL 说明书 1

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

CLIA Kit for Connective Tissue Growth Factor (CTGF)

实验原理

将结缔组织生长因子(CTGF)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的结缔组织生长因子(CTGF)与连接于固相载体上的抗体结合,然后加入生物素化的结缔组织生长因子(CTGF)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的结缔组织生长因子(CTGF)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA010Ra01 结缔组织生长因子(CTGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA010Ra01 结缔组织生长因子(CTGF)多克隆抗体 WB; IHC; ICC; IP.
LAA010Ra81 结缔组织生长因子(CTGF)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA010Ra71 结缔组织生长因子(CTGF)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA010Ra21 结缔组织生长因子(CTGF)单克隆抗体 WB; IHC; ICC; IP.
LAA010Ra72 结缔组织生长因子(CTGF)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA010Ra 结缔组织生长因子(CTGF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA010Ra 结缔组织生长因子(CTGF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
PSA010Ra01 结缔组织生长因子(CTGF)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA010Ra01 结缔组织生长因子(CTGF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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World Journal of Gastroenterology Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell function [PubMed: PMC2726461]
PLos One Activation of JNK Signaling Mediates Connective Tissue Growth Factor Expression and Scar Formation in Corneal Wound Healing [Plosone: 0032128]
Clinical Biochemistry Clinical significance of inflammatory and fibrogenic cytokines in diabetic nephropathy [ScienceDirect: S0009912012000987]
The Journal of Heart and Lung Transplantation Connective tissue growth factor (CTGF/CCN2): A protagonist in cardiac allograft vasculopathy development? [PubMed: S1053249812010121]
Biomedical and Environmental Sciences The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS Rats [Besjournal: Source]
Disease Markers Concentrations of connective tissue growth factor in patients with nonalcoholic fatty liver disease: Association with liver fibrosis [PubMed: 22846210]
World Journal of Gastroenterology Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosis [PubMed: PMC3351780]
Journal of ethnopharmacology Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. [Pubmed: 24211397]
Radiation Oncology Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapy [Ro-journal: Source]
PLoS One. Knock Out of S1P3 Receptor Signaling Attenuates Inflammation and Fibrosis in Bleomycin-Induced Lung Injury Mice Model [Pubmed:Pmc4157792]
European Journal of Heart Failure Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan Diastolic Heart Failure Registry and molecular basis by cellular and animal models [Pubmed:24464932]
Bone Connective Tissue Growth Factor is a Target of Notch Signaling in Cells of the Osteoblastic Lineage [Pubmed:24792956]
American Journal of Pathology Disruption of collagen homeostasis can reverse established age-related myocardial fibrosis [Pubmed:25701883]
J Gastroenterol Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitis [PubMed: 26190501]
Life Sciences Suppression of connective tissue growth factor mediates the renoprotective effect of Sitagliptin rather than Pioglitazone in type 2 diabetes mellitus [Pubmed:27049870]
International Journal of Molecular Sciences TGF-β and Physiological Root Resorption of Deciduous Teeth. [pubmed:28035998]
Inflammation Research Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211]
Heart & Vessels Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175]
British Journal of Ophthalmology The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidence [pubmed:27531356]
Pathophysiology Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseases [pubmed:27686729]
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