褪黑素(MT)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Melatonin (MT)

N-Acetyl-5-Methoxytryptamine

  • 褪黑素(MT)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 褪黑素(MT)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 褪黑素(MT)检测试剂盒(化学发光免疫分析法) 实验结果图
  • CCA908Ge.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测褪黑素(MT),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的褪黑素(MT)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 85-102 98
EDTA plasma(n=5) 85-101 92
heparin plasma(n=5) 97-104 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的褪黑素(MT),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中褪黑素(MT)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 96-104% 94-105% 96-103% 88-99%
EDTA plasma(n=5) 83-101% 98-105% 91-105% 92-99%
heparin plasma(n=5) 79-92% 80-93% 87-96% 89-103%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加底物100µL,37°C孵育10分钟;
7. 读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将褪黑素(MT)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的褪黑素(MT)呈负相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA908Ge11 褪黑素(MT)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA908Ge21 褪黑素(MT)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA908Ge01 褪黑素(MT)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MEA908Ge 褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA908Ge 褪黑素(MT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA908Ge 褪黑素(MT)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA908Ge 褪黑素(MT)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA908Ge 褪黑素(MT)单因子检测试剂盒(流式荧光发光法) Magnetic Luminex Assay for Antigen Detection.
KSA908Ge11 褪黑素(MT)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Domestic Animal Endocrinology Effects of melatonin on islet neogenesis and beta cell apoptosis in streptozotocin-induced diabetic rats: an immunohistochemical study [ScienceDirect: S0739724012000112]
General Assembly and Scientific Symposium Biomarkers inducing changes due to microwave exposure effect on rat brain [Academin: Source]
Chinese Journal Comparison of the flow characteristics for the honeycomb and labyrinth seal using numerical simulation [Cnki: Source]
Clinics (Sao Paulo). The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave field [PubMed: 21876981]
Journal of Radiation Research Effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on serum hormone levels in rats [PubMed: 23239176]
Experimental Gerontology Improvement of oxidative stress and immunity by melatonin: An age dependent study in golden hamster [Pubmed: 23220117]
Journal of Human Reproductive Sciences Melatonin and its correlation with testosterone in polycystic ovarian syndrome [NCBI: PMC3963309]
Indian Journal of Experimental Biology Daily variation in melatonin level, antioxidant activity and general immune response of peripheral blood mononuclear cells and lymphoid tissues of Indian goat Capra &hellip; [Pubmed: 24851409]
Metabolic Syndrome Serum Melatonin Level Disturbance is Related to Metabolic Syndrome and Subclinical Arterial Dysfunction in Shift Working Healthy Men [Researchgate: Source]
Israel Journal of Veterinary Medicine Melatonin Administration Aids Bone Healing in Diabetic Rats [Ijvm: Source]
Journal of Photochemistry and Photobiology B: Biology Photoperiodic induced melatonin regulates immunity and expression pattern of melatonin receptor MT1 in spleen and bone marrow mononuclear cells of male golden hamster. [Pubmed: 24090924]
In Vivo. Influence of?circadian?time?and lighting conditions on expression of melatonin?receptors?1?and?2?in?murinelymphocytes. [Pubmed:25189896]
Biomicrofluidics. Molecularly imprinted electrochemical sensing of urinary melatonin in a microfluidic system [Pubmed:25584113]
International Journal of Advanced Research Localization and expression of androgen receptor (AR) and melatonin membrane receptor (MT1R) in accessory sex organs of adult male golden hamster, Mesocricetus auratus: Modulation by photoperiod. [Com:Source]
Journal of Nanoparticle Research Titanium oxide (TiO2) nanoparticles in induction of apoptosis and inflammatory response in brain [Springer:Source]
International Immunopharmacology MT1 receptor expression and AA-NAT activity in lymphatic tissue following melatonin administration in male golden hamster [Pubmed:25023124]
Journal of Photochemistry and Photobiology B: Biology Photoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratus [Pubmed:25255424]
Andrologia Melatonin prevents dexamethasone-induced testicular oxidative stress and germ cell apoptosis in golden hamster, Mesocricetus auratus [Pubmed:25277062]
Biological Rhythm Research Reproductive seasonality in the Indian pygmy field mouse, Mus terricolor [Tandfonline:Source]
Acta Histochem Developmental changes of melatonin receptor expression in the spleen of the chicken, Gallus domesticus [PubMed: 26024931]
Acta Histochemica Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks [Pubmed:26948666]
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers [Pubmed:27051212]
Journal of Photochemistry and Photobiology B: Biology Effect of melatonin on monochromatic light-induced T-lymphocyte proliferation in the thymus of chickens [Pubmed:27203566]
Asian-Australas J Anim Sci. Effect of Monochromic Light-emitting Diode Light with Different Color on the Growth and ReproductivePerformances of Breeder Geese. [Pubmed:26954165]
Revista Brasileira de Saúde e Produção Animal Desempenho e comportamento de suínos em fase de terminação submetidos a diferentes programas de luz [S1519-99402016000200272]
journal of neuroendocrinology Green light inhibits GnRH-I expression by stimulating the melatonin-GnIH pathway in the chick brain. [pubmed:28295740]
Revista Brasileira de Medicina do Esporte EFFECTS OF DIURNAL AND NOCTURNAL STRENUOUS EXERCISE ON SERUM MELATONIN LEVELS [S1517-86922016000600436]
Journal of Photochemistry and Photobiology B Role of monochromatic light on daily variation of clock gene expression in the pineal gland ofchick. [pubmed:27643985]
Journal of Oral Science Antioxidant effects of melatonin in heart tissue after induction of experimental periodontitis inrats. [pubmed:28367898]
畜牧兽医学报 单色绿光对鸡胚褪黑激素合成的影响 [DOI: 10.11843/j.issn.0366-6964.2017.05.019]
Oxidative Medicine and Cellular Longevity Melatonin Supplementation Lowers Oxidative Stress and Regulates Adipokines in Obese Patients on a Calorie-Restricted Diet [pubmed:29142618]
Pakistan veterinary journal Inhibitory Effect of Melatonin on Formation of the Chicken Primordial Follicles. [ISSN: 0253-8318]
Acta histochemica Melatonin modulates monochromatic light-induced melatonin receptor expression in the hypothalamus of chicks [pubmed:28943001]
Stem Cell Reviews and Reports A Circadian Rhythm in both Complement Cascade (ComC) Activation and Sphingosine-1-Phosphate (S1P) Levels in Human Peripheral Blood Supports a … [10.1007:s12015-018-9836-7]
Photochemistry and Photobiology Effect of Monochromatic Light on Circadian Rhythm of Clock Genes in Chick Pinealocytes [Pubmed:29896808]
Reproduction In Domestic Animals Expression of melatonin and its related synthase and membrane receptors in the oestrous corpus luteum and corpus luteum verum of sheep [Pubmed:29943511]
OCCUPATIONAL HYGIENE–PERFORMANCE COFACTOR IN POWER ENGINEERING. BIOCHEMICAL RESPONSE TO EXPOSURE TO EXTREMELY LOW … [id:PLUMEE201601V06S01A0004]
Asian Spine Journal A Comparative Analysis of the Metabolic and Coagulative Profiles in Patients with Idiopathic Scoliosis, Congenital Scoliosis and Healthy Controls: A Case … [Pubmed: 30322254]
Endocrine Connections cBmal1 but not cClock is associated with monochromatic green light-induced circadian rhythm of melatonin in chick pinealocytes [Pubmed: 30533004]
Reproductive biology and endocrinology Profile of melatonin and its receptors and synthesizing enzymes in cumulus–oocyte complexes of the developing sheep antral follicle—a potential estradiol … [Pubmed: 30606208]
Chronobiology international Effect of pinealectomy on the circadian clock of the chick retina under different monochromatic lights [Pubmed: 30663441]
Journal of Pineal Research Plasma levels of melatonin in dilated cardiomyopathy [Pubmed: 30715754]
Journal of Clinical Medicine Elevated Serum Melatonin under Constant Darkness Enhances Neural Repair in Spinal Cord Injury through Regulation of Circadian Clock Proteins Expression [Pubmed: 30678072]
Brain, Behavior, and Immunity Melatonergic agonist regulates circadian clock genes and peripheral inflammatory and neuroplasticity markers in patients with depression and anxiety []
International Journal of Molecular Sciences Mel1c Mediated Monochromatic Light-Stimulated IGF-I Synthesis through the Intracellular Gαq/PKC/ERK Signaling Pathway [Pubmed: 30987295]
Journal of Pineal Research Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267]
PLoS One Melatonin mediates monochromatic green light-induced satellite cell proliferation and muscle growth in chick embryo [Pubmed: 31059537]
Immunology Letters Effect of the melatonin nuclear receptor RORα on monochromatic light-induced T-lymphocyte proliferation in chicken thymus [Pubmed: 31326445]
Journal of photochemistry and photobiology. B, Biology Effect of melatonin on monochromatic light-induced changes in clock gene circadian expression in the chick liver [Pubmed: 31247384]
Life sciences Melatonin maintains mitochondrial membrane potential and decreases excessive intracellular Ca2+ levels in immature human oocytes [Pubmed: 31472147]
Journal of Clinical Medicine Conditional Controlled Light/Dark Cycle Influences Exercise-Induced Benefits in a Rat Model with Osteoarthritis: In Vitro and In Vivo Study [Pubmed: 31684092]
experimental eye research Effect of monochromatic light on the circadian clock of cultured chick retinal tissue [Pubmed: 32198015]
Medicine Saliva cortisol, melatonin levels and circadian rhythm alterations in Chinese primary school children with dyslexia [Pubmed: 32028434]
pharmaceutical biology Role of melatonin in intestinal mucosal injury induced by restraint stress in mice [Pubmed: 32298156]
FISH PHYSIOLOGY AND BIOCHEMISTRY Hypoxia-mediated inhibition of cholesterol synthesis leads to disruption of nocturnal sex steroidogenesis in the gonad of koi carp, Cyprinus carpio [Pubmed: 33034795]
INFLAMMATION RESEARCH Sleep deprivation aggravated lipopolysaccharide/d-galactosamine-induced acute liver injury by suppressing melatonin production [Pubmed: 32809047]
Journal of Health Sciences and Medicine Effects of tryptophan, a precursor for melatonin, on IVF outcomes and Doppler parameters []
急性睡眠剥夺对小鼠血浆褪黑激素水平和下丘脑钟基因表达的影响 []
Influence of phytomelatonin on immune cell function in male golden hamsters []
Horm Mol Biol Clin Investig Investigation of the effect of training on serotonin, melatonin and hematologic parameters in adolescent basketball players [34090322]
Frontiers in Cell and Developmental Biology Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats [33644068]
Oxidative Medicine and Cellular Longevity A Green and Blue Monochromatic Light Combination Therapy Reduces Oxidative Stress and Enhances B-Lymphocyte Proliferation through Promoting ¡­ [33828639]
International Journal of General Medicine Identification of Serotonin as a Predictive Marker for Breast Cancer Patients [34045888]
Physiological crosstalk between the Mel1a and Mel1c pathways modulates melatonin-mediated, monochromatic light combination-induced bursa B-lymphocyte?¡­ []
Exp Neurol MiR-375-3p mediates reduced pineal function in hypoxia-ischemia brain damage [34280452]
Oxid Med Cell Longev Parameters of Oxidative Stress, Vitamin D, Osteopontin, and Melatonin in Patients with Lip, Oral Cavity, and Pharyngeal Cancer [34721756]
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