骨桥素(OPN)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Osteopontin (OPN)

SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

  • 骨桥素(OPN)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 骨桥素(OPN)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 骨桥素(OPN)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA899Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测骨桥素(OPN),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的骨桥素(OPN)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 84-92 87
EDTA plasma(n=5) 90-101 97
heparin plasma(n=5) 80-104 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的骨桥素(OPN),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中骨桥素(OPN)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 79-89% 95-102% 99-105% 81-92%
EDTA plasma(n=5) 81-90% 81-101% 90-104% 94-101%
heparin plasma(n=5) 89-97% 94-101% 95-102% 90-97%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

实验原理

将骨桥素(OPN)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的骨桥素(OPN)与连接于固相载体上的抗体结合,然后加入生物素化的骨桥素(OPN)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的骨桥素(OPN)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA899Mu01 骨桥素(OPN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA899Mu61 骨桥素(OPN)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA899Mu02 骨桥素(OPN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA899Mu01 骨桥素(OPN)多克隆抗体 WB; IHC; ICC; IP.
PAA899Mu02 骨桥素(OPN)多克隆抗体 WB; IHC; ICC; IP.
PAA899Mu06 骨桥素(OPN)多克隆抗体 WB; IHC; ICC; IP.
SEA899Mu 骨桥素(OPN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA899Mu 骨桥素(OPN)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA899Mu01 骨桥素(OPN)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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The Journal of Rheumatology Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis. [Jrheum: source]
International Journal of Pharmaceutics Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester) [ScienceDirect: S0378517312003742]
European Cells and matreials The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering [Ecmjournal: Source]
Journal of Equine Veterinary Science Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse [ScienceDirect: Source]
Basic &amp; Clinical Pharmacology &amp; Toxicology Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions [Wiley:Source]
Journal of Cellular Biochemistry Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts [Pubmed:24905552]
Naunyn-Schmiedeberg&#039;s Archives of Pharmacology Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats [Pubmed:25038619]
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Gene Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis [PubMed: 26283150]
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370]
Journal of Biomaterials and Tissue Engineering Osteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based Hydrogel [Content: Asp]
Primasari Osteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancer [Index.Php: Amor]
Int J Biol Sci Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway [PubMed: 26327817]
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma. [Ebscohost]
Am J Pathol.  Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. [Pubmed:26963343]
circulation journal Stenotic Bicuspid and Tricuspid Aortic Valves - Micro-Computed Tomography and Biological Indices of Calcification. [pubmed:28344201]
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American Journal of Translational Research Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes. [pubmed:28337262]
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