睾酮(Testo)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Testosterone (Testo)

T; Testesteron; Testeronun

  • 睾酮(Testo)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 睾酮(Testo)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 睾酮(Testo)检测试剂盒(化学发光免疫分析法) 实验结果图
  • CCA458Ge.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测睾酮(Testo),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的睾酮(Testo)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 94-102 97
EDTA plasma(n=5) 78-101 85
heparin plasma(n=5) 96-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的睾酮(Testo),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中睾酮(Testo)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 84-101% 90-104% 88-95% 84-94%
EDTA plasma(n=5) 81-101% 84-104% 85-99% 91-105%
heparin plasma(n=5) 84-97% 78-102% 78-91% 89-98%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加底物100µL,37°C孵育10分钟;
7. 读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将睾酮(Testo)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的睾酮(Testo)呈负相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA458Ge11 睾酮(Testo)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA458Ge21 睾酮(Testo)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA458Ge01 睾酮(Testo)多克隆抗体 IHC; ICC; IP.
LAA458Ge71 睾酮(Testo)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA458Ge21 睾酮(Testo)单克隆抗体 WB; IHC; ICC; IP.
IEA458Ge 睾酮(Testo)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA458Ge 睾酮(Testo)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA458Ge 睾酮(Testo)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA458Ge 睾酮(Testo)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA458Ge11 睾酮(Testo)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".
KSA458Ge12 睾酮(Testo)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
The Tohoku Journal of Experimental Medicine Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature Rats [Jstage: source]
Plos one Insulin Receptor Substrate 2 Is Required for Testicular Development [PubMed: PMC3669358]
International Journal of Molecular and Cellular Medicine Central effects of camphor on GnRH and sexual hormones in male rat [Pubmed: 24551777]
Fertility and Sterility Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells [Pubmed: 23954356]
Urologia internationalis Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats [Pubmed: 24051982]
International Braz J Urol The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats. [Pubmed: 24642156]
Journal of Materials Chemistry B Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering [Rsc: Source]
Biochemical and Biophysical Research Communications Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis [ScienceDirect: S0006291X14001843]
PLoS One. Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostate [Pubmed:Pmc4100933]
Reproductive Toxicology Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring [Pubmed:24126190]
Physiology &amp; Behavior The impact of maternal consumption of cafeteria diet on reproductive function in the offspring [Pubmed:24631302]
Int Braz J Urol. The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in rats [Pubmed:24642156]
Environmental Research Perfluorooctane sulfonate effects on the reproductive axis in adult male rats [Pubmed:25171141]
Acta Cir Bras. Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model. [Pubmed:25185055]
Genet Mol Biol. Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility [Pubmed:25505846]
Turkish Journal of Urology Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy [Turkishjournalofurology:Source]
Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes [PubMed: 26379831]
Int Braz J Urol Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult Rats [PubMed: 26200553]
Advances in Environmental Biology Plasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic Extract [Aensiweb: Aeb]
international journal of environmental research and public health Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy [PubMed: 25996886]
Environ Toxicol Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period [PubMed: 25782374]
J Endocrinol Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction [PubMed: 25770118]
Acta Pharmaceutica Sinica B Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrh [science:S2211383516301812]
Plos One Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota [Pubmed:27093642]
Theriogenology Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle count [Pubmed:26948295]
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα [Pubmed:27413362]
TURKISH JOURNAL OF MEDICAL SCIENCES THE EFFECT OF KISSPEPTIN ON SPERMATOGENESIS AND APOPTOSIS IN RATS [fileID:693211]
Int J Mol Sci. Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice [Pubmed:26771609]
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373]
Journal of Applied Life Sciences International Maternal Sleep Deprivation Alters Reproductive Capability of Male Offspring in Wistar Rats [Akindele922016JALSI29782.pdf]
Integrative Zoology Recovery of fertility in quinestrol-treated or diethylstilbestrol-treated mice: Implications for rodent management. [pubmed:27611741]
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389]
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. [pubmed:27838061]
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα [pubmed:27413362]
Andrologia Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. [pubmed:28000943]
Turkish journal of medical sciences The effect of kisspeptin on spermatogenesis and apoptosis in rats. [pubmed:28263511]
Kafkas Universitesi Veteriner Fakultesi Dergisi The Angiogenic Effect of Testosterone Supplementation on Brain of Aged Mice. [13006045]
Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitium [S2405580816300991]
Reproductive Sciences Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome [pubmed:28673204]
Biomedicine & Pharmacotherapy Toxic effects of Tripterygium wilfordii Hook F on the reproductive system of adolescent male rats [pubmed:28938525]
Molecular and Cellular Endocrinology Testosterone deficiency prevents left ventricular contractility dysfunction after myocardial infarction [pubmed:28606867]
Ankara Üniv Vet Fak Derg The effect of testosterone supplementation on morphometry and VEGF expression level of thoracic aorta in aged mice [DOI: 10.1501/Vetfak_0000002791]
Acta histochemica Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-) [pubmed:28506466]
DARU Journal of Pharmaceutical Sciences Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway [pubmed:29020999]
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625]
Aquatic Toxicology Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations [Pubmed:29775925]
Molecular Reproduction and Development  deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and … [Pubmed:29411926]
Journal of Ovarian Research DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway [Pubmed:29321035]
Medical Science Monitor Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) … [Pubmed:29627845]
Oncology Letters Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes … [10.3892:ol.2018.9165]
留言咨询