雄烯二酮(ASD)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Androstenedione (ASD)

4-Androstenedione; 4-Androstene-3,17-Dione

  • 雄烯二酮(ASD)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 雄烯二酮(ASD)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对雄烯二酮(ASD)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

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PAA456Ge01 雄烯二酮(ASD)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
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CEA456Ge 雄烯二酮(ASD)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
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LMA456Ge 雄烯二酮(ASD)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA456Ge11 雄烯二酮(ASD)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Plos One Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota [Pubmed:27093642]
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373]
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389]
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146]
International Journal of Biological Sciences Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells []
Molecular reproduction and development Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries [Pubmed: 31490589]
DOMESTIC ANIMAL ENDOCRINOLOGY Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells [Pubmed: 32739762]
ANIMAL REPRODUCTION SCIENCE A study on steroidogenic elaborations of stroma and their regulation in response to ovarian hormones in goats [33845412]
Reprod Domest Anim Extracellular Vesicles of Bovine Small Follicular Fluid Promote Ovarian Cortical Stromal Cell Proliferation and Steroidogenesis [34402549]
Curr Oncol Plasma Aromatase Activity Index, Gonadotropins and Estrone Are Associated with Frailty Syndrome in Post-Menopausal Women with Breast Cancer [Pubmed:35323344]
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