血管紧张素原(AGT)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Angiotensinogen (AGT)

ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)

  • 血管紧张素原(AGT)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管紧张素原(AGT)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对血管紧张素原(AGT)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
APA797Ra61 血管紧张素原(AGT)活性蛋白 Cell culture; Activity Assays.
RPA797Ra01 血管紧张素原(AGT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA797Ra61 血管紧张素原(AGT)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA797Ra01 血管紧张素原(AGT)多克隆抗体 WB; IHC; ICC; IP.
LAA797Ra71 血管紧张素原(AGT)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA797Ra81 血管紧张素原(AGT)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA797Ra21 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
MAA797Ra22 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
MAA797Ra23 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
MAA797Ra24 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
MAA797Ra27 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
MAA797Ra26 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
SEA797Ra 血管紧张素原(AGT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA797Ra 血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA797Ra01 血管紧张素原(AGT)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Amyloid The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patients [PubMed: 22320202]
Pediatric Mephrology Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertension [PubMed: PMC3661928]
Acta Paediatrica Urinary angiotensinogen as a novel marker of obstructive nephropathy in children [Pubmed: 23772991]
Journal of the Renin-Angiotensin-Aldosterone System Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia [Sagepub: Source]
Pediatric Nephrology MP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRY [Oxfordjournals:Source]
journal of human hypertension Relationship between blood pressure var [PubMed: 26223347]
PLoS One Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes Analysis [PubMed: 26098644]
MKA Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasa [Index.Php: Art]
Journal of Human Hypertension Relationship between blood pressure variability and renal activity of the renin-angiotensin system. [pubmed:26223347]
Acta Pharmacol Sin. Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. [pubmed:27890915]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
Life Sciences Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity [Pubmed:30036488]
American Journal of Physiology-Renal Physiology Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats [Pubmed:29846109]
Methods in Molecular Biology Quantitation of Total and Free Thiol Angiotensinogen as a Prognostic Marker for Preeclampsia [Pubmed: 31069778]
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555]
Hypertens Res Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents [33568792]
Physiol Rep (Pro) renin receptor antagonist PRO20 attenuates nephrectomy©\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/¦Â©\catenin signaling [34057312]
Cancer Cell Int A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer [34215253]
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