白介素35(IL35)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Interleukin 35 (IL35)

  • 白介素35(IL35)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 白介素35(IL35)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 白介素35(IL35)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品图片
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对白介素35(IL35)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPC008Hu01 白介素35(IL35)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC008Hu01 白介素35(IL35)活性蛋白 Cell culture; Activity Assays.
PAC008Hu01 白介素35(IL35)多克隆抗体 WB; IHC; ICC; IP.
MAC008Hu21 白介素35(IL35)单克隆抗体 WB; IHC; ICC; IP.
LAC008Hu72 白介素35(IL35)单克隆抗体(生物素标记) WB; IHC; ICC.
SEC008Hu 白介素35(IL35)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEC008Hu 白介素35(IL35)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
PSC008Hu01 白介素35(IL35)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSC008Hu01 白介素35(IL35)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Oncology Letters Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia. [PubMed: 22783403]
Journal of Maternal-Fetal and Neonatal Medicine Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease [Pubmed: 24175856]
Journal of Reproductive Immunology What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? [Pubmed: 24368037]
Human Immunology The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia [ScienceDirect: S0198885913005776]
Folia Biologica (Praha) Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers [Pubmed:24785110]
Molecular Medicine Reports Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis [Pubmed:25323532]
Rom J Morphol Embryol. The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy [Pubmed:25611266]
Journal of Immunotherapy Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity [PubMed: 25962106]
PLOS ONE Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis [PubMed: 26204444]
Rheumatology (Oxford) Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease [PubMed: 26231346]
Cytokine Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia [PubMed: 26282938]
J Biol Chem Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway [PubMed: 26085094]
Br J Haematol Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia [PubMed: 25640666]
Autoimmunity Reviews New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literature [Pubmed:26876383]
Clinical research Increased plasma concentrations of interleukin 35 in patients with coronary artery disease [Journal:-19]
Clinical Rheumatology The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis [pubmed:27502600]
Respiratory Medicine Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis [pubmed:27492538]
International Journal of Molecular Medicine Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines. [pubmed:27460435]
上海口腔医学 IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析 [QK:98604X]
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