丙二醛(MDA)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Malondialdehyde (MDA)

  • 丙二醛(MDA)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 丙二醛(MDA)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对丙二醛(MDA)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA597Ge41 丙二醛(MDA)人血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA597Ge11 丙二醛(MDA)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA597Ge21 丙二醛(MDA)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA597Ge01 丙二醛(MDA)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA597Ge24 丙二醛(MDA)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA597Ge23 丙二醛(MDA)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA597Ge22 丙二醛(MDA)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA597Ge21 丙二醛(MDA)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEA597Ge 丙二醛(MDA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA597Ge 丙二醛(MDA)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA597Ge 丙二醛(MDA)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA597Ge 丙二醛(MDA)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA597Ge11 丙二醛(MDA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Cell stress chaperones Lipopolysaccharide pretreatment protects against ischemia/reperfusion injury via increase of HSP70 and inhibition of NF-κB [PubMed: 21562784]
Liver transplantation Effect of intermittent hepatic inflow occlusion with the Pringle maneuver during donor hepatectomy in adult living donor liver transplantation with right hemiliver grafts: a prospective, randomized controlled study [PubMed: 21837746]
Journal of Molecular Neuroscience Formaldehyde Impairs Learning and Memory Involving the Disturbance of Hydrogen Sulfide Generation in the Hippocampus of Rats [PubMed: 23108488]
Hepatogastroenterology The amelioration of AH by abstinence and the attenuation of oxidative stress [PubMed: 21940381]
Clinical and Experimental Hypertension High salt intake accelerated cardiac remodeling in spontaneously hypertensive rats: time window of left ventricular functional transition and its relation to salt-loading doses [PubMed: 21529316]
AJBM Effect of caffeic acid on doxorubicin induced cardiotoxicity in rats [Ajbm: Source]
Yonsei Med J. Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients. [Pubmed: 24142649]
Cardiovasc Pathol.? Chronic thromboembolic pulmonary hypertension is not associated with iron overload [Pubmed:25449047]
Bosn J Basic Med Sci. Oxidative stress status in elite athletes engaged in different sport disciplines [Pubmed:Pmc4333958]
Neurochemical Research Role of Aldehyde Dehydrogenase 2 in 1-Methy-4-Phenylpyridinium Ion-Induced Aldehyde Stress and Cytotoxicity in PC12 Cells [Pubmed:25005621]
Stroke.? Ischemic postconditioning relieves cerebral ischemia and reperfusion injury through activating T-LAK cell-originated protein kinase/protein kinase B pathway in rats. [Pubmed:25013016]
Mol Med Rep Metformin alleviates high glucose [PubMed: 25760137]
Mediators Inflamm. Influence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic Syndrome [PubMed: 26538831]
PLOS ONE BDNF-TrkB Pathway Mediates Neuroprotection of Hydrogen Sulfide against Formaldehyde-Induced Toxicity to PC12 Cells [PubMed: 25749582]
Mediators of Inflammation Influence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic Syndrome [PubMed: 26538831]
Sci Rep Mitochondrial respiratory dysfunctions of blood mononuclear cells link with cardiac disturbance in patients with early-stage heart failure [PubMed: 26018291]
Nutrition Obstructive sleep apnea is independently associated with inflammation and insulin resistance, but not with blood pressure, [PubMed: 26429654]
Med Sci Monit Puerarin Attenuates Anoxia/Reoxygenation Injury Through Enhancing Bcl-2 Associated Athanogene 3 Expression, a Modulator of Apoptosis and Autophagy [Pubmed:27011313]
BMC Complement Altern Med Mechanism (s) of action underlying the gastroprotective effect of ethyl acetate fraction obtained from the crude methanolic leaves extract of Muntingia calabura [Pubmed:26912079]
Toxicology Mechanisms and Methods Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanism [pubmed:27599793]
International Journal of Neuropsychopharmacology Hydrogen Sulfide Ameliorates Homocysteine-Induced Cognitive Dysfunction by Inhibition of Reactive Aldehydes Involving Upregulation of ALDH2 [pubmed:27988490]
Revista de Chimie -Bucharest Comparative Analysis of Some Antioxidant Markers in Periodontal Disease [pdf:MARTU%207%2016.pdf]
打不开网页 Influence of Supplementing Diet with Microalgae (Schizochytrium limacinum) on Growth and Metabolism of Lambs during the Summer 2 [180761]
Cellular Physiology and Biochemistry Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy [pubmed:27889753]
Chinese Journal of Integrative Medicine Panax notoginseng saponins protect kidney from diabetes by up-regulating silent informationregulator 1 and activating antioxidant proteins in rats. [pubmed:26712211]
Experimental Cell Research Hydrogen sulfide inhibits MPP+-induced aldehyde stress and endoplasmic reticulum stress inPC12 cells: involving upregulation of BDNF. [pubmed:27641114]
Anim Reprod Sci. Transcriptional abundance of antioxidant enzymes in endometrium and their circulating levels in Zebu cows with and without uterine infection. [pubmed:28007408]
Journal of Cancer Research and Treatment Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokines [jcrt:5]
Clin Exp Pharmacol Physiol. Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. [pubmed:28251688]
Turkish Journal of Veterinary and Animal Sciences Influence of supplementing diet with microalgae (Schizochytrium limacinum) on growth and metabolism in lambs during the summer [795296]
Oncotarget Hydrogen sulfide attenuates chronic restrain stress-induced cognitive impairment by upreglulation of Sirt1 in hippocampus [pubmed:29245987]
Experimental and Therapeutic Medicine Resveratrol attenuates type 2 diabetes mellitus by mediating mitochondrial biogenesis and lipid metabolism via Sirtuin type 1 [10.3892/etm.2017.5400]
Pharmacological Reports Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without posttraumatic stress disorder [10.1016/j.pharep.2017.08.008]
Journal of Pain Research Sex differences in complex regional pain syndrome type I (CRPS-I) in mice [pubmed:28831269]
Environmental toxicology and pharmacology Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stress [pubmed:28654832]
Frontiers in Microbiology Burn Serum Increases Staphylococcus aureus Biofilm Formation via Oxidative Stress. [pubmed:28702016]
Ecotoxicology and Environmental Safety Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oil [Pubmed:29660615]
Biomed Research International Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway [Pubmed:29770336]
Toxicologic Pathology Gold Nanoparticle Toxicity in Mice and Rats: Species Differences [Pubmed:29742986]
Molecular Medicine Reports Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion [Pubmed:29393473]
Molecular Medicine Reports  Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781]
Molecular Medicine Reports Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathway [Pubmed:29620209]
Oncology Letters Hydrogen‑rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus‑activated kinase 2/signal transducer and activator of … [Pubmed:29928398]
Human and Ecological Risk Assessment: An International Journal Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snails [10.1080:10807039.2018.1460800]
Experimental and Therapeutic Medicine Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway [Pubmed:30116359]
Biochemistry and Biotechnology Research Lead-induced increase in erythrocyte osmotic fragility and malondialdehyde concentration in Wistar rats: Chemo-protective effects of flavonoid mixture [2018/1/17-021.pdf]
BMC Pharmacology and Toxicology Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis [Pubmed:29321061]
Medical Journal Armed Forces India Are thalassemia patients oxidatively challenged? [10.1016:j.mjafi.2017.12.002]
Arquivos Brasileiros de Cardiologia Serum Uric Acid Levels are Associated with Cardiometabolic Risk Factors in Healthy Young and Middle-Aged Adults [Pubmed: 30328946]
Clinical and Translational Oncology Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1 [Pubmed: 30284230]
BMC Complementary and Alternative Medicine  Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents [Pubmed: 28095910]
Journal of Pharmacological Sciences Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome [Pubmed: 30342783]
Biochemical and Biophysical Research Communications Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats [Pubmed: 30454888]
Journal of Integrative Medicine Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats [Doi: 10.1016/j.joim.2018.12.002]
Stem Cells Translational Medicine Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats [Pubmed: 30569668]
Evidence-Based Complementary and Alternative Medicine Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway []
Examination of Oxidative Stress Markers in Women with Postmenopausal Osteoporosis []
Acta clinica Croatica The Relationship Between Serum Level of Malondialdehyde and Progression of Colorectal Cancer []
Journal of Pediatric Surgery The effects of hydrogen-rich saline solution on intestinal anastomosis performed after intestinal ischemia reperfusion injury []
European Journal of Biomedical and Pharmaceutical Sciences European Journal of Biomedical AND Pharmaceutical sciences []
Orphanet journal of rare diseases The cardiovascular phenotype of adult patients with phenylketonuria [Pubmed: 31492166]
Neuropsychobiology Hydrogen Sulfide Ameliorates Cognitive Dysfunction in Formaldehyde-Exposed Rats: Involvement in the Upregulation of Brain-Derived Neurotrophic Factor [Pubmed: 31550727]
Alcohol. Influence of deficient nutrition on trace element status and antioxidant defense system [Pubmed: 1781925]
Journal of Controlled Release Ginsenoside Rg3-loaded, reactive oxygen species-responsive polymeric nanoparticles for alleviating myocardial ischemia-reperfusion injury [Pubmed: 31783047]
Antioxidants Phenolic Compounds and the Anti-Atherogenic Effect of Bee Bread in High-Fat Diet-Induced Obese Rats [Pubmed: 31905919]
Frontiers in Neuroscience Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the Hippocampus [Pubmed: 32218719]
PLoS One NRF2 deficiency increases obesity susceptibility in a mouse menopausal model [Pubmed: 32045430]
Medicina Selected Oxidative Stress Markers in Colorectal Cancer Patients in Relation to Primary Tumor Location—A Preliminary Research [Pubmed: 31972987]
Stem Cells International Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … [Pubmed: 32300366]
Sci Rep NFKB1 gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han population [Pubmed: 33177541]
LIFE SCIENCES Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathway [Pubmed: 32798557]
Oxidative Medicine and Cellular Longevity Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics [Pubmed: 32566106]
Food and Agricultural Immunology Effects of microencapsulated probiotics and plant extract on antioxidant ability, immune status and caecal microflora in Escherichia coli K88-challenged broiler … []
Possible Role of Statins on the Inflammatory Biomarkers in Patients With Periodontal Disease: A Cross-Sectional Study []
Tualang honey improves memory and hippocampal changes in adult offspring of prenatally stressed rats []
Malaysian Journal of Medicine and Health Sciences Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring []
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING The Anticarcinogenic Effect of the Apple Polyphenol Phloretin in an Experimental Rat Model of Hepatocellular Carcinoma []
Toxicology Formaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effect [33259821]
Turk J Pharm Sci Tualang Honey Improves Memory and Prevents Hippocampal Changes in Prenatally Stressed Rats [33389951]
Biosci Rep Endostatin attenuates heart failure via inhibiting reactive oxygen species in myocardial [32686821]
Stem Cell Research & Therapy Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis [33413675]
J Pediatr Urol Protective effects of hydrogen rich saline solution on ventral penile Mathieu type flap with penile tourniquet application in rats [33608226]
Mol Med Rep Tanshinone IIA attenuates heart failure via inhibiting oxidative stress in myocardial infarction rats [33786621]
Amino Acids Alarin alleviated cardiac fibrosis via attenuating oxidative stress in heart failure rats [34089389]
Exp Ther Med Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats [34093766]
Experimental and Therapeutic Medicine MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma [33732346]
The Influence of Probiotic Supplementation on Depressive Symptoms, Inflammation and Oxidative Stress Parameters and Faecal Microbiota in Patients with Depression Depending on Metabolic Syndrome Comorbidity ¨C PRO-DEMET Randomised Study Protocol [33804999]
J Healthc Eng κ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB Pathway [34608407]
Cell Death Discov Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats [Pubmed:35022384]
Sleep Breath Peripheral blood oxidative stress markers for obstructive sleep apnea—a meta-analysis [Pubmed:34981298]
J Dairy Sci Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver disease [Pubmed:35086715]
Peptides Fibroblast growth factor 12 attenuated cardiac remodeling via suppressing oxidative stress [Pubmed:35304156]
Peptides Targeting of midkine alleviates cardiac hypertrophy via attenuation of oxidative stress and autophagy [Pubmed:35427698]
Antioxidants Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice [Pubmed:35740008]
J Inflamm Res The κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in Rats [Pubmed:35645576]
BioMed Research International Malondialdehyde and Neutrophil Gelatinase-Associated Lipocalin as Markers of Oxidative Stress in Small for Gestational Age Newborns from Hypertensive … [Pubmed:35224102]
Association of Oxidative Stress Markers with Vascular Stiffness Parameters in Patients with Diabetic Neuropathy []
Stem Cell Research & Therapy Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model []
留言咨询