基质金属蛋白酶1(MMP1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Matrix Metalloproteinase 1 (MMP1)

CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase

  • 基质金属蛋白酶1(MMP1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 基质金属蛋白酶1(MMP1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对基质金属蛋白酶1(MMP1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Parasitology Research Therapeutic effect of alpha lipoic acid combined with praziquantel on liver fibrosis induced by Schistosoma mansoni challenged mice [SpringerLink: j33n80517577535k]
Infection and Immunity Bovine Respiratory Syncytial Virus and Histophilus somni Interaction at the Alveolar Barrier [Pubmed: 23649093]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655]
The Journal of Pathology Mycobacterium tuberculosis dysregulates MMP/TIMP balance to drive rapid cavitation and unrestrained bacterial proliferation [Pubmed:25186281]
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747]
J Immunol Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis [PubMed: 26268658]
Cellular & Molecular Immunology Effect of ebosin on modulating interleukin-1&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes [PubMed: 25938977]
J Periodontal Implant Sci Assessment of MMP-1, MMP-8 and TIMP-2 in experimental periodontitis treated with kaempferol [pmc:PMC4848383]
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. [Pubmed:26991748]
Cellular & Molecular Immunology Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes [pubmed:25938977]
Molecular Medicine Reports Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells [pubmed:28447732]
Analytical Methods The development of a matrix metalloproteinase-1 biosensor based on the surface plasmon resonance imaging technique [c6ay01856d]
Journal of Cellular Physiology Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models [pubmed:27739057]
Journal of Applied Animal Research Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208]
Analytical Methods SPRI biosensors for quantitative determination of matrix metalloproteinase-2 [DOI:10.1039/C7AY00786H]
International Wound Journal From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases [pubmed:26991748]
European Heart Journal Biomarker‐based phenotyping of myocardial fibrosis identifies patients with heart failure with preserved ejection fraction resistant to the beneficial effects of … [Pubmed:29709099]
Journal of Agricultural and Food Chemistry Hawthorn polyphenol extract inhibits UVB-induced skin photoaging by regulating MMP expression and type I procollagen production in mice [Pubmed:30032605]
Oral Diseases  TIMP‐1 association with collagen type I overproduction in hereditary gingival fibromatosis [Pubmed:29989318]
Journal of Applied Animal Research  Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis [10.1080:09712119.2017.1335208]
PLoS One Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis [Pubmed: 30939166]
МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ []
International Journal of Molecular Sciences A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor [Pubmed: 31052424]
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY MT 1‐MMP evaluation in neointimal hyperplasia in the late follow‐up after prosthesis implantation []
Journal of Plastic, Reconstructive & Aesthetic Surgery Intraluminal fluid infusion in a rat jejunum ischemia/reperfusion model is associated with improved tissue perfusion and less mucosal damage [Pubmed: 31734236]
Bone Research Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis [Pubmed: 32047705]
Scientific Reports Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722]
JOURNAL OF ETHNOPHARMACOLOGY Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling … [Pubmed: 32745509]
Food Funct Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice [33890588]
Phytomedicine Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK … [Pubmed:35660352]
Journal of Immunology Research Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes [Pubmed:35340587]
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