95 KSA528Hu01 | 血小板衍生生长因子A(PDGFA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Homo sapiens (Human,人) USCN(武汉优尔生商贸有限公司)

血小板衍生生长因子A(PDGFA)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Platelet Derived Growth Factor Subunit A (PDGFA)

PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide

  • 血小板衍生生长因子A(PDGFA)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 血小板衍生生长因子A(PDGFA)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对血小板衍生生长因子A(PDGFA)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA528Hu01 血小板衍生生长因子A(PDGFA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA528Hu01 血小板衍生生长因子A(PDGFA)活性蛋白 Cell culture; Activity Assays.
LAA528Hu71 血小板衍生生长因子A(PDGFA)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA528Hu81 血小板衍生生长因子A(PDGFA)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA528Hu22 血小板衍生生长因子A(PDGFA)单克隆抗体 WB; IHC; ICC; IP.
MAA528Hu21 血小板衍生生长因子A(PDGFA)单克隆抗体 WB; IHC; ICC; IP.
SEA528Hu 血小板衍生生长因子A(PDGFA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA528Hu 血小板衍生生长因子A(PDGFA)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA528Hu01 血小板衍生生长因子A(PDGFA)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Canadian Journal of Physiology and Pharmacology The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680]
International Immunopharmacology Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway [pubmed:28521243]
Open Access Macedonian Journal of Medical Sciences Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells []
Prostaglandins & other Lipid Mediators Lipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathway [Pubmed: 31698141]
Ann Agric Environ Med PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis [Pubmed: 32208584]
Tissue Eng Regen Med Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method [34339025]
Biomed Research International Thermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like Form [Pubmed:35586817]
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