细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Programmed Cell Death Protein 1 Ligand 1 (PDL1)

CD274; PDCD1LG1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1

  • 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品图片
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对细胞程序性死亡蛋白1配体1(PDCD1LG1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA788Hu01 细胞程序性死亡蛋白1配体1(PDL1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA788Hu61 细胞程序性死亡蛋白1配体1(PDL1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA788Hu01 细胞程序性死亡蛋白1配体1(PDL1)多克隆抗体 WB; IHC; ICC; IP.
FAA788Hu01 抗细胞程序性死亡蛋白1配体1(PDL1)多克隆抗体 Flow cytometry.
FAA788Hu41 抗细胞程序性死亡蛋白1配体1(PDL1)多克隆抗体(藻红素标记) Flow cytometry.
FAA788Hu51 抗细胞程序性死亡蛋白1配体1(PDL1)多克隆抗体(别藻蓝蛋白标记) Flow cytometry.
FAA788Hu81 抗细胞程序性死亡蛋白1配体1(PDL1)多克隆抗体(异硫氰酸荧光素标记) Flow cytometry.
MAA788Hu22 细胞程序性死亡蛋白1配体1(PDL1)单克隆抗体 WB; IHC; ICC; IP.
MAA788Hu21 细胞程序性死亡蛋白1配体1(PDL1)单克隆抗体 WB; IHC; ICC; IP.
LAA788Hu72 细胞程序性死亡蛋白1配体1(PDL1)单克隆抗体(生物素标记) WB; IHC; ICC.
WEA788Hu 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA788Hu 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA788Hu 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
PSA788Hu01 细胞程序性死亡蛋白1配体1(PDL1)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA788Hu01 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Chinese Journal of Cancer Level of circulating PD-L1 expression in patients with advanced gastric cancer and its clinical implications [NCBI: PMC3937742]
Leukemia.? High level of soluble programmed cell death ligand 1 in blood impacts overall survival in aggressive diffuse large B-Cell lymphoma: results from a French multicenter clinical trial. [Pubmed:24732592]
Diabetes?Metab Res Rev. PD‐L1 gene polymorphisms and low serum level of PD‐L1 protein are associated to type 1 diabetes in Chile [Pubmed:24816853]
Lancet Haematol Ratios of T-cell immune effectors and checkpoint molecules as prognostic biomarkers in diffuse large B-cell lymphoma: a population-based study [PubMed: 26686046]
Eur J Cancer.  High levels of the soluble programmed death-ligand (sPD-L1) identify hepatocellular carcinoma patients with a poor prognosis. [Pubmed:27039170]
Journal of Cancer Research and Clinical Oncology Serum levels of soluble programmed cell death ligand 1 as a prognostic factor on the first-line treatment of metastatic or recurrent gastric cancer [article:10.1007]
Journal of Hematology & Oncology PD-L1 is upregulated by EBV-driven LMP1 through NF-κB pathway and correlates with poor prognosis in natural killer/T-cell lymphoma [pubmed:27737703]
Lung Cancer. High plasma levels of soluble programmed cell death ligand 1 are prognostic for reduced survival in advanced lung cancer. [pubmed:28212990]
Oncotarget Soluble programmed death-ligand 1 (sPDL1) and neutrophil-to-lymphocyte ratio (NLR) predictssurvival in advanced biliary tract cancer patients treated with palliative chemotherapy. [pubmed:27780932]
Oncotarget High post-treatment serum levels of soluble programmed cell death ligand 1 predict earlyrelapse and poor prognosis in extranodal NK/T cell lymphoma patients. [pubmed:27105512]
Oncotarget.  Soluble PD-L1: A biomarker to predict progression of autologous transplantation in patients with multiple myeloma [pubmed:27566569]
Cancer Immunol Immunother. Programmed death-ligand 1 and its soluble form are highly expressed in nasal natural killer/T-cell lymphoma: a potential rationale for immunotherapy. [pubmed:28349165]
Leukemia Soluble programmed death-ligand 1 as a prognostic biomarker for overall survival in patients with diffuse large B-cell lymphoma: a replication study and combined analysis of 508 patients [leu2016385a]
Medicine Plasma levels of soluble programmed death ligand-1 may be associated with overall survival in nonsmall cell lung cancer patients receiving thoracic radiotherapy. [pubmed:28207525]
Allergology International  Inverse correlation of soluble programmed cell death-1 ligand-1 (sPD-L1) with eosinophil count and clinical severity in allergic rhinitis patients [pubmed:27617656]
Medicine Plasma levels of soluble programmed death ligand-1 may be associated with overall survival in nonsmall cell lung cancer patients receiving thoracic radiotherapy [PMC5319514]
留言咨询