细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Programmed Cell Death Protein 1 Ligand 1 (PDL1)

CD274; PDCD1LG1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1

  • 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品图片
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对细胞程序性死亡蛋白1配体1(PDCD1LG1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA788Hu01 细胞程序性死亡蛋白1配体1(PDL1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA788Hu01 细胞程序性死亡蛋白1配体1(PDL1)多克隆抗体 WB; IHC; ICC; IP.
FAA788Hu01 抗细胞程序性死亡蛋白1配体1(PDL1)多克隆抗体 Flow cytometry.
FAA788Hu41 抗细胞程序性死亡蛋白1配体1(PDL1)多克隆抗体(藻红素标记) Flow cytometry.
FAA788Hu51 抗细胞程序性死亡蛋白1配体1(PDL1)多克隆抗体(别藻蓝蛋白标记) Flow cytometry.
FAA788Hu81 抗细胞程序性死亡蛋白1配体1(PDL1)多克隆抗体(异硫氰酸荧光素标记) Flow cytometry.
MAA788Hu22 细胞程序性死亡蛋白1配体1(PDL1)单克隆抗体 WB; IHC; ICC; IP.
WEA788Hu 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA788Hu 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA788Hu 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA788Hu01 细胞程序性死亡蛋白1配体1(PDL1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Chinese Journal of Cancer Level of circulating PD-L1 expression in patients with advanced gastric cancer and its clinical implications [NCBI: PMC3937742]
Leukemia.? High level of soluble programmed cell death ligand 1 in blood impacts overall survival in aggressive diffuse large B-Cell lymphoma: results from a French multicenter clinical trial. [Pubmed:24732592]
Diabetes?Metab Res Rev. PD‐L1 gene polymorphisms and low serum level of PD‐L1 protein are associated to type 1 diabetes in Chile [Pubmed:24816853]
Lancet Haematol Ratios of T-cell immune effectors and checkpoint molecules as prognostic biomarkers in diffuse large B-cell lymphoma: a population-based study [PubMed: 26686046]
Eur J Cancer.  High levels of the soluble programmed death-ligand (sPD-L1) identify hepatocellular carcinoma patients with a poor prognosis. [Pubmed:27039170]
Journal of Cancer Research and Clinical Oncology Serum levels of soluble programmed cell death ligand 1 as a prognostic factor on the first-line treatment of metastatic or recurrent gastric cancer [article:10.1007]
Journal of Hematology & Oncology PD-L1 is upregulated by EBV-driven LMP1 through NF-κB pathway and correlates with poor prognosis in natural killer/T-cell lymphoma [pubmed:27737703]
Lung Cancer. High plasma levels of soluble programmed cell death ligand 1 are prognostic for reduced survival in advanced lung cancer. [pubmed:28212990]
Oncotarget Soluble programmed death-ligand 1 (sPDL1) and neutrophil-to-lymphocyte ratio (NLR) predictssurvival in advanced biliary tract cancer patients treated with palliative chemotherapy. [pubmed:27780932]
Oncotarget High post-treatment serum levels of soluble programmed cell death ligand 1 predict earlyrelapse and poor prognosis in extranodal NK/T cell lymphoma patients. [pubmed:27105512]
Oncotarget.  Soluble PD-L1: A biomarker to predict progression of autologous transplantation in patients with multiple myeloma [pubmed:27566569]
Cancer Immunol Immunother. Programmed death-ligand 1 and its soluble form are highly expressed in nasal natural killer/T-cell lymphoma: a potential rationale for immunotherapy. [pubmed:28349165]
Leukemia Soluble programmed death-ligand 1 as a prognostic biomarker for overall survival in patients with diffuse large B-cell lymphoma: a replication study and combined analysis of 508 patients [leu2016385a]
Medicine Plasma levels of soluble programmed death ligand-1 may be associated with overall survival in nonsmall cell lung cancer patients receiving thoracic radiotherapy. [pubmed:28207525]
Allergology International  Inverse correlation of soluble programmed cell death-1 ligand-1 (sPD-L1) with eosinophil count and clinical severity in allergic rhinitis patients [pubmed:27617656]
留言咨询