95 KSB622Ra01 | 表面活性物质关联蛋白B(SP-B)检测试剂盒DIY材料(酶联免疫吸附试验法) | Rattus norvegicus (Rat，大鼠) USCN(武汉优尔生商贸有限公司)

表面活性物质关联蛋白B(SP-B)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Surfactant Protein B (SP-B)

SFTPB; PSPB; SFTB3; SFTP3; SPB; Surfactant Associated Protein B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein

  • 表面活性物质关联蛋白B(SP-B)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 表面活性物质关联蛋白B(SP-B)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对表面活性物质关联蛋白B(SPB)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB622Ra01 表面活性物质关联蛋白B(SP-B)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB622Ra01 表面活性物质关联蛋白B(SP-B)多克隆抗体 WB; IHC; ICC; IP.
MAB622Ra21 表面活性物质关联蛋白B(SP-B)单克隆抗体 WB; IHC; ICC; IP.
SEB622Ra 表面活性物质关联蛋白B(SP-B)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB622Ra 表面活性物质关联蛋白B(SP-B)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB622Ra01 表面活性物质关联蛋白B(SP-B)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Respiratory Physiology & Neurobiology Kinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgery [PubMed: 21736957]
American Journal of Rhinology & Allergy Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps [PubMed: 23406594]
19 Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot study [PubMed: 20650982]
Plos one Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins [PubMed: PMC3551896]
Plos one The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS [PubMed: PMC3787032]
Cancer Cell. Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor Models [PubMed: PMC3406925]
Respir Physiol Neurobiol Acute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps project [Pubmed: 23619193]
PLOS ONE Surfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart Failure [Pubmed:25514679]
Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge [Opus4:Source]
Int J Cardiol Plasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective study [PubMed: 26310985]
Plos One Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) [Pubmed:27337142]
PLoS One The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions. [pubmed:27656877]
Frontiers in Aging Neuroscience Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid. [pubmed:28101052]
Frontiers in Aging Neuroscience Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid [PMC5209370]
Respiratory Physiology & Neurobiology Diving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional divers [pubmed:28467885]
Cellular Physiology and Biochemistry Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production [pubmed:28743125]
American Journal of Physiology. Lung Cellular and Molecular Physiology Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. [pubmed:29167126]
Respiratory research Effects and molecular mechanisms of intrauterine infection/inflammation on lung development [Pubmed:29747649]
Respiratory Physiology & Neurobiology  Surfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with Levosimendan [Pubmed:29548887]
Lung  Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation [Pubmed:29445934]
American Journal of Physiology Lung Cellular andMolecular Physiology Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells [Pubmed: 30211653]
Respiratory Research Culture of human alveolar epithelial type II cells by sprouting [Pubmed: 30340591]
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