紧密连接蛋白1(TJP1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Tight Junction Protein 1 (TJP1)

ZO1; Zona Occludens 1; Zona occludens protein 1; Zonula occludens protein 1

  • 紧密连接蛋白1(TJP1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 紧密连接蛋白1(TJP1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对紧密连接蛋白1(TJP1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Current Nutrition & Food Science Barrier Strengthening and Anti-inflammatory Effect of Cucurbit Fruits on Intestinal Epithelial Cells In-vitro [10.2174:1573401313666170427123153]
Journal of Translational Medicine Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation [Pubmed: 33036625]
Neural Regeneration Research Inhibition of nitric oxide synthase aggravates brain injury in diabetic rats with traumatic brain injury [33433486]
PLoS One A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and … [34492054]
J Agric Food Chem Pyocyanin Modulates Gastrointestinal Transformation and Microbiota [Pubmed:35171599]
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