三碘甲状腺原氨酸(T3)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Triiodothyronine (T3)

3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

  • 三碘甲状腺原氨酸(T3)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 三碘甲状腺原氨酸(T3)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对三碘甲状腺原氨酸(T3)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA453Ge11 三碘甲状腺原氨酸(T3)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
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MAA453Ge22 三碘甲状腺原氨酸(T3)单克隆抗体 WB; IHC; ICC; IP.
LAA453Ge72 三碘甲状腺原氨酸(T3)单克隆抗体(生物素标记) WB; IHC; ICC.
CEA453Ge 三碘甲状腺原氨酸(T3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA453Ge 三碘甲状腺原氨酸(T3)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA453Ge 三碘甲状腺原氨酸(T3)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA453Ge11 三碘甲状腺原氨酸(T3)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Aquatic Toxicology Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae [ScienceDirect: S0166445X12000215]
Aquatic Toxicology Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis [PubMed: 23220413]
European Journal of Zoological Research Effect of dietary tryptophan on plasma growth hormone and thyroid hormone in broiler chicks [Scholarsresearchlibrary: Source]
Life Science Journal Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies [Lifesciencesite: Source]
PLoS One A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism [Pubmed: 23468891]
Aquat Toxicol Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay. [ScienceDirect: S0166445X13002129]
Aquatic Toxicology Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae [Pubmed:25456233]
Chemosphere Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam Ruditapes philippinarum [science:S0045653516302016]
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Sci Rep Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. [pubmed:28322267]
Aquat Toxicol. Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus). [pubmed:28187359]
Bulletin of the World Health Organization Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders [handle:123456789]
Biological Rhythm Research Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes [09291016.2016.1275393]
Toxicological Sciences Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction [pubmed:28973666]
Environmental Pollution Transgenerational endocrine disruption and neurotoxicity in zebrafish larvae after parental exposure to binary mixtures of decabromodiphenyl ether (BDE-209) and lead [10.1016/j.envpol.2017.06.053]
Sciencepaper online 四溴双酚A对菲律宾蛤仔的生长抑制效应机理Growth inhibition Effects of TBBPA on juvenile manila clam Ruditapes philippinarum [5790267088906950000]
Tropical Journal of Pharmaceutical Research Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats [10.4314/tjpr.v16i5.9]
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s-space Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult [10371/137708]
Environmental Pollution Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish. [pubmed:28624628]
Environmental Science & Technology Thyroid hormone-disrupting potentials of major benzophenones in two cell lines (GH3 and FRTL-5) and embryo-larval zebrafish [Pubmed:29995391]
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625]
Toxicological Sciences Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci]
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355]
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