酪氨酸羟化酶(TH)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Tyrosine Hydroxylase (TH)

TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14

  • 酪氨酸羟化酶(TH)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 酪氨酸羟化酶(TH)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对酪氨酸羟化酶(TH)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPB438Hu01 酪氨酸羟化酶(TH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB438Hu01 酪氨酸羟化酶(TH)多克隆抗体 WB; IHC; ICC; IP.
MAB438Hu21 酪氨酸羟化酶(TH)单克隆抗体 WB; IHC; ICC; IP.
MAB438Hu24 酪氨酸羟化酶(TH)单克隆抗体 WB; IHC; ICC; IP.
MAB438Hu25 酪氨酸羟化酶(TH)单克隆抗体 WB; IHC; ICC; IP.
MAB438Hu22 酪氨酸羟化酶(TH)单克隆抗体 WB; IHC; ICC; IP.
SEB438Hu 酪氨酸羟化酶(TH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB438Hu 酪氨酸羟化酶(TH)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSB438Hu01 酪氨酸羟化酶(TH)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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PLOS ONE Genetic Diagnosis of Two Dopa-Responsive Dystonia Families by Exome Sequencing [Pubmed:25181484]
biomedicine & pharmacotherapy Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883]
Therapeutics and Clinical Risk Management Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population. [pubmed:27540296]
Sci Rep.  Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. [pubmed:28230187]
Journal of Cellular Biochemistry Vaccarin administration ameliorates hypertension and cardiovascular remodeling in renovascular hypertensive rats [pubmed:28681939]
Environmental Science & Technology Multigenerational disruption of the thyroid endocrine system in marine medaka after a life-cycle exposure to perfluorobutanesulfonate [Pubmed:29565584]
Molecular Neurobiology Cilostazol Mediated Nurr1 and Autophagy Enhancement: Neuroprotective Activity in Rat Rotenone PD Model [Pubmed:29429051]
Chemosphere Tetrabromobisphenol A caused neurodevelopmental toxicity via disrupting thyroid hormones in zebrafish larvae [Pubmed:29407805]
Neurospine The Effect of Recombinant Tyrosine Hydroxylase Expression on the Neurogenic Differentiation Potency of Mesenchymal Stem Cells [Pubmed:29656620]
Toxicology and Applied Pharmacology Montelukast attenuates rotenone-induced microglial activation/p38 MAPK expression in rats: Possible role of its antioxidant, anti-inflammatory and antiapoptotic effects [Pubmed: 30222980]
International Journal Of Cardiology Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus [Pubmed: 30581101]
Behavioural Brain Research Hydroxychloroquine antiparkinsonian potential: Nurr1 modulation versus autophagy inhibition []
Environment International Perfluoropolyether carboxylic acids (novel alternatives to PFOA) impair zebrafish posterior swim bladder development via thyroid hormone disruption [Pubmed: 31733528]
Environmental Pollution Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvae [Pubmed: 31935612]
International Journal of Pharmacology Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's disease [Pubmed: 32084576]
comparative biochemistry and physiology c-toxicology & pharmacology Thyroid disruption and developmental toxicity caused by Cd2+ in Schizopygopsis younghusbandi larvae [Pubmed: 32344129]
International Journal of Stem cell Research & Therapy Selection of Cells for Parkinson's Disease Cell-Therapy []
Ecotoxicol Environ Saf Effects of SiO2 nanoparticles on the uptake of tetrabromobisphenol A and its impact on the thyroid endocrine system in zebrafish larvae [33385677]
Nutr Res Tocotrienols protect differentiated SH-SY5Y human neuroblastoma cells against 6-hydroxydopamine-induced cytotoxicity by ameliorating dopamine biosynthesis and … [Pubmed:35065349]
Food Chem Toxicol Omarigliptin attenuates rotenone-induced Parkinson's disease in rats: Possible role of oxidative stress, endoplasmic reticulum stress and immune modulation [Pubmed:35439590]
Food Chemistry: Molecular Sciences Unravelling the neuroprotective mechanisms of carotenes in differentiated human neural cells: biochemical and proteomic approaches [Pubmed:35415676]
Journal of Environmental Sciences Parental and transgenerational impairments of thyroid endocrine system in zebrafish by 2, 4, 6-tribromophenol []
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