1,25-二羟基维生素D3(DHVD3)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for 1,25-Dihydroxyvitamin D3 (DHVD3)
DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3
- 编号CEA467Ge
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围24.69-2,000pg/mL
- 灵敏度最小可检测剂量小于等于9.92pg/mL.
- 样本类型Serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3342 ¥ 4774 ¥ 21481 ¥ 40576 ¥ 334152
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测1,25-二羟基维生素D3(DHVD3)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的1,25-二羟基维生素D3(DHVD3)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 95-104 | 101 |
| EDTA plasma(n=5) | 83-95 | 87 |
| heparin plasma(n=5) | 86-98 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的1,25-二羟基维生素D3(DHVD3)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中1,25-二羟基维生素D3(DHVD3)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 81-92% | 88-98% | 89-96% |
| EDTA plasma(n=5) | 87-102% | 92-101% | 92-99% | 92-101% |
| heparin plasma(n=5) | 78-89% | 93-101% | 78-103% | 96-103% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将DHVD3单克隆抗体包被微孔板，制成固相载 体，往包被抗体的微孔中同时加入生物素标记的DHVD3类似物和待测抗原(标准品或样本)，待测抗原与生物素标记DHVD3类似物对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CEA467Ge | 1,25-二羟基维生素D3(DHVD3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA467Ge | 1,25-二羟基维生素D3(DHVD3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
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| Tumor Biology | Calcitriol?induced?redox?imbalance and DNA breakage in cells sharing a common metabolic feature of malignancies: Interaction with cellular copper (II) ions leads to the production of reactive oxygen species. [Pubmed:25547436] |
| International Journal of Life Sciences Research | Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats [profile:303311214] |
| Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats [:] | |
| Apoptosis | Calcitriol–copper interaction leads to non enzymatic, reactive oxygen species mediated DNA breakage and modulation of cellular redox scavengers in hepatocellular … [article:10.1007] |
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| International Journal of Clinical and Experimental Medicine | Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats [] |
| Journal of Clinical and Translational Endocrinology | Dobesity: relationship between vitamin d deficiency, obesity and sclerostin as a novel biomarker of bone metabolism [] |
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| Ecotoxicology and Environmental Safety | Sodium dehydroacetate induces cardiovascular toxicity associated with Ca2+ imbalance in zebrafish [] |
| EXPERIMENTAL AND MOLECULAR MEDICINE | Metabolic activities affect femur and lumbar vertebrae remodeling, and anti-resorptive risedronate disturbs femoral cortical bone remodeling [33436949] |
| Life Sci | SWIM domain protein ZSWIM4 is required for JAK2 inhibition resistance in breast cancer [34102191] |
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