5-羟色胺(5-HT)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for 5-Hydroxytryptamine (5-HT)

5HT; Serotonin; N-Methyl-Gamma

  • 5-羟色胺(5-HT)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 5-羟色胺(5-HT)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 5-羟色胺(5-HT)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA808Ge.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测5-羟色胺(5-HT),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的5-羟色胺(5-HT)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-91 81
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 89-102 95

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的5-羟色胺(5-HT),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中5-羟色胺(5-HT)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 78-97% 90-102% 78-102% 78-97%
EDTA plasma(n=5) 80-95% 78-89% 92-105% 78-98%
heparin plasma(n=5) 91-104% 78-103% 80-99% 84-95%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将5-羟色胺(5-HT)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA808Ge11 5-羟色胺(5-HT)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA808Ge21 5-羟色胺(5-HT)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA808Ge01 5-羟色胺(5-HT)多克隆抗体 IHC; ICC; IP.
LAA808Ge71 5-羟色胺(5-HT)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA808Ge81 5-羟色胺(5-HT)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
CEA808Ge 5-羟色胺(5-HT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA808Ge 5-羟色胺(5-HT)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA808Ge11 5-羟色胺(5-HT)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Fitoterapia Ginsenoside rich fraction of Panax ginseng C.A. Meyer improve feeding behavior following radiation-induced pica in rats [ScienceDirect: S0367326X12001153]
Clinical & Experimental Allergy Sensitization induces hypersensitivity in trigeminal nerve [PubMed: 23106664]
International Journal of Immunopathology & Pharmacology Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats [PubMed: 26684638]
Neuropsychiatr Dis Treat Catha edulis chewing effects on treatment of paranoid schizophrenic patients [PubMed: 25926735]
Neuroscience Letters Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression [Pubmed:26773866]
Gene  Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE [Pubmed:27196063]
American Journal of Animal and Veterinary Sciences Metabolic Features of Heart Failure with Different Etiology [ofsp11075]
Medical Science Monitor Basic Research Valproate Attenuates Nitroglycerin-Induced Trigeminovascular Activation by Preserving Mitochondrial Function in a Rat Model of Migraine [pubmed:27618395]
Molecular Medicine Reports PI3K/AKT signaling pathway activation in a rat model of migraine. [pubmed:28791398]
Neuropsychiatric disease and treatment The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic … [Pubmed:29379286]
Evidence-Based Complementary and Alternative Medicine Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids [Pubmed:29853946]
Andrologia Changes in sexual performance and biochemical characterisation of functional neural regions: A study in serotonin transporter knockout male rats [Pubmed: 31037750]
Neuroscience Serotonin-Mediated Cardiac Analgesia Via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction [Pubmed: 31153963]
留言咨询