激活素A(ACVA)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Activin A (ACVA)

ACV-A; Activin Beta A Beta A Homodimer

  • 激活素A(ACVA)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 激活素A(ACVA)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 激活素A(ACVA)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA001Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测激活素A(ACVA),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的激活素A(ACVA)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 85-103 99
EDTA plasma(n=5) 91-98 94
heparin plasma(n=5) 95-103 98

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的激活素A(ACVA),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中激活素A(ACVA)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 95-102% 92-101% 80-103%
EDTA plasma(n=5) 93-102% 91-98% 95-103% 88-102%
heparin plasma(n=5) 98-105% 87-101% 79-99% 96-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将激活素A(ACVA)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
CEA001Ra 激活素A(ACVA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
13 Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte Dysfunction [Ahajournals: Source]
J. Pharmacogn [Article: Hksobf_2015_V46N2_167]
United States Patent Application Methods for Diagnosis and Treatment of Concussion or Brain Injury [2016:0103140]
BMC Cancer. Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. [pubmed:27835986]
Basic Clin Pharmacol Toxicol. Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. [pubmed:28032440]
Basic & Clinical Pharmacology & Toxicology Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling [doi:10.1111]
Journal of Immunology MicroRNA-27b Modulates Inflammatory Response and Apoptosis during Mycobacterium tuberculosis Infection [Pubmed:29661829]
Journal of Vascular Surgery Expression of platelet-derived growth factor B is upregulated in patients with thoracic aortic dissection [Pubmed:29685513]
Acta Biochimica et Biophysica Sinica Toxic effects of arsenic trioxide on protoscoleces through ROS production, and Ca-ER stress-dependent apoptosis [10.1093:abbs]
Poultry Science The effect of raw and fermented rapeseed cake on the metabolic parameters, immune status, and intestinal morphology of turkeys [Pubmed:29917099]
Food Bioscience Characterization of Phospholipids from Pacific Saury () Viscera and Their Neuroprotective Activity [10.1016:j.fbio.2018.06.002]
Current Analytical Chemistry Single Wall Carbon Nanotube and Magnetic Bead Based Electrochemical Immunosensor for Sensitive Detection of Salivary Secretory Immunoglobulin A [10.2174:2211556006666170525161225]
Journal of Veterinary Internal Medicine FRIDAY, NOVEMBER 3, 2017 11.30-12.0 BALLROOM AB [:]
Universal Journal of Pharmaceutical Research SEROPREVALENCE OF ANTI-MANNOSE BINDING LECTIN AUTOANTIBODIES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY-YEMEN [10.22270:ujpr.v3i2.138]
Involvement of RBP4 in diabetic atherosclerosis and the role of vitamin D intervention [:]
Clin Oral Investig.  Gingival crevicular fluid levels of human beta-defensin-1 in type 2 diabetes mellitus and periodontitis [Pubmed:29713888]
Myocardial fibrosis in hypertrophic cardiomyopathy [:]
TELLING TRANSVERSAL COMPETENCES… TO BE PROFESSIONALLY PROMOTED [:]
Protein biomarker panels for detecting colorectal cancer and advanced adenoma [:]
Endothelotropic activity of 4-hydroxy-3, 5-di-tret-butylcinnamic acid in the conditions of experimental cerebral ischemia [:]
Silencing of the CSNK2β gene by siRNA inhibits invasiveness and growth of MDA-MB-231 cells [10.1101:311092]
Duchenne Muscular Dystrophy Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular Dystrophy [Pubmed:29067667]
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Journal of Geriatric Oncology Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors [Pubmed: 30952517]
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