抗促性腺激素释放激素抗体(Anti-GnRH)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH)

GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

  • 抗促性腺激素释放激素抗体(Anti-GnRH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 抗促性腺激素释放激素抗体(Anti-GnRH)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 抗促性腺激素释放激素抗体(Anti-GnRH)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • AEA843Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测抗促性腺激素释放激素抗体(Anti-GnRH),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的抗促性腺激素释放激素抗体(Anti-GnRH)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 80-99 87
heparin plasma(n=5) 87-101 95

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的抗促性腺激素释放激素抗体(Anti-GnRH),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中抗促性腺激素释放激素抗体(Anti-GnRH)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 79-92% 88-95% 97-105% 80-95%
EDTA plasma(n=5) 79-102% 90-97% 79-99% 78-98%
heparin plasma(n=5) 82-97% 80-93% 98-105% 88-95%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板5次;
5. 加TMB底物90µL,37°C孵育10-20分钟;
6. 加终止液50µL,立即450nm读数。

实验原理

将抗原包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的抗促性腺激素释放激素抗体(Anti-GnRH)与连接于固相载体上的抗原结合,然后加入HRP标记的二抗,彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗促性腺激素释放激素抗体(Anti-GnRH)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA843Mu01 促性腺激素释放激素(GnRH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA843Mu02 促性腺激素释放激素(GnRH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA843Mu01 促性腺激素释放激素(GnRH)多克隆抗体 WB; IHC; ICC; IP.
PAA843Mu02 促性腺激素释放激素(GnRH)多克隆抗体 WB; IHC; ICC; IP.
LAA843Mu71 促性腺激素释放激素(GnRH)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA843Mu81 促性腺激素释放激素(GnRH)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
CEA843Mu 促性腺激素释放激素(GnRH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA843Mu 抗促性腺激素释放激素抗体(Anti-GnRH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antibody Detection.
CCA843Mu 促性腺激素释放激素(GnRH)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA843Mu11 促性腺激素释放激素(GnRH)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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