过氧化氢酶(CAT)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Catalase (CAT)

  • 过氧化氢酶(CAT)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 过氧化氢酶(CAT)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 过氧化氢酶(CAT)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEC418Bo.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测过氧化氢酶(CAT)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的过氧化氢酶(CAT)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 91-105 99
EDTA plasma(n=5) 81-97 85
heparin plasma(n=5) 92-101 95

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的过氧化氢酶(CAT)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中过氧化氢酶(CAT)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 94-101% 86-94% 78-90%
EDTA plasma(n=5) 86-101% 78-92% 92-101% 79-101%
heparin plasma(n=5) 97-105% 90-99% 91-98% 89-103%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将过氧化氢酶(CAT)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的过氧化氢酶(CAT)与连接于固相载体上的抗体结合，然后加入生物素化的过氧化氢酶(CAT)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的过氧化氢酶(CAT)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Bos taurus; Bovine (Cattle，牛) 应用(仅供研究使用，不用于临床诊断！)
NPC418Bo01 过氧化氢酶(CAT)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC418Bo01 过氧化氢酶(CAT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC418Bo02 过氧化氢酶(CAT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC418Bo05 过氧化氢酶(CAT)多克隆抗体 WB; IHC; ICC; IP.
SEC418Bo 过氧化氢酶(CAT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Arteriosclerosis, Thrombosis, and Vascular Biology Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic Aneurysm [Ahajournals: source]
Fundam Clin Pharmacol. Phloroglucinol protects gastric mucosa against ethanol-induced injury through regulating myeloperoxidase and catalase activities [PubMed: 20880383]
Oral Disease Antioxidant profile of salivary glands in high fat diet-induced insulin resistance rats [Pubmed: 24106991]
Yonsei Med J. Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients. [Pubmed: 24142649]
Journal of Food Science and Technology Grape seed extract and Zinc containing nutritional food supplement delays onset and progression of Streptozocin-induced diabetic cataract in Wistar rats [Springer: Source]
第 三 军 医 大 学 学 报 过氧化氢酶基因 ３８９Ｃ＞Ｔ多态位点在重庆汉族人群中的分布及其与噪音性耳聋的 关联研究 [Tmmu:Source]
Adv Med Sci Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, [PubMed: 26674569]
Arch Oral Biol Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes [PubMed: 26143097]
J Oral Pathol Med Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva [PubMed: 26608886]
О Р И Г И Н А Л Ь Н Ы Е С Т А Т Ь И КЛИНИЧЕСКОЕ ЗНАЧЕНИЕ ФАКТОРОВ КЛЕТОЧНОЙ РЕГУЛЯЦИИ И ФЕРМЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ У ПАЦИЕНТОК РЕПРОДУКТИВНОГО ВОЗРАСТА С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИ [docs:225188]
Current Alzheimer Research Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients. [pubmed:27748187]
Journal of Cancer Research and Treatment Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokines [jcrt:5]
Adv Med Sci. Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects. [pubmed:28242484]
PeerJ Laminarin protects against hydrogen peroxide-induced oxidative damage in MRC-5 cells possibly via regulating NRF2 [pubmed:28785522]
Pharmacological Reports Evaluation of ADMA, carbonyl groups, CAT and NKA in depressed patients with and without posttraumatic stress disorder. [pubmed:28554099]
Ecotoxicology and Environmental Safety Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oil [Pubmed:29660615]
Human and Ecological Risk Assessment: An International Journal Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snails [10.1080:10807039.2018.1460800]
Experimental and Therapeutic Medicine Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway [Pubmed:30116359]
Journal of Cosmetic Dermatology A red pumpkin seed extract reduces melanosome transfer to keratinocytes by activation of Nrf2 signaling [Pubmed:30047218]
Anticancer Research Cholecystectomy Patients with High Plasma Level of Catalase Have Significantly Lower Analgesia Requirement: A Prospective Study of Two Different … [Pubmed: 30194197]
Anticancer Research Plasma Catalase in Relation to Pain Following Midline Laparotomy: A Prospective Study of Patients with Benign Diseases and Patients with Cancer [Pubmed: 30396975]
Journal of Integrative Medicine Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats [Doi: 10.1016/j.joim.2018.12.002]
EXPERIMENTAL ANIMALS Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formation [Pubmed: 30606939]
MOLECULAR BIOLOGY REPORTS An in vitro protocol to study the effect of hyperglycemia on intracellular redox signaling in human retinal pigment epithelial (ARPE-19) cells [Pubmed: 30628023]
Anticancer Research Nitrotyrosine (NT), a Nitrosative Stress Biomarker, Plasma Concentrations in Gallstone Disease and Cancer Patients [Pubmed: 30711961]
Anticancer Research Rectus Sheath Block (RSB) Analgesia Could Enhance Significantly the Patient Satisfaction Following Midline Laparotomy in Benign Disease and in Cancer: A … [Pubmed: 30842172]
Molecular Medicine Reports MicroRNA‑21 contributes to the puerarin‑induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injury [Pubmed: 31115556]
Advances in Medical Sciences Selected elements of extracellular matrix of the skin in diabetes and insulin resistance [Pubmed: 31146169]
European Journal of Biomedical and Pharmaceutical Sciences European Journal of Biomedical AND Pharmaceutical sciences []
Anticancer Research The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer [Pubmed: 31892574]
PHARMACOLOGICAL RESEARCH A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 Signaling [Pubmed: 32135248]
Biomolecules Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development [Pubmed: 32751983]
AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS []
The Journal of Physiology In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells []
Journal of Cell Communication and Signaling Lutein reverses hyperglycemia-mediated blockage of Nrf2 translocation by modulating the activation of intracellular protein kinases in retinal pigment epithelial (ARPE … []
Plasma catalase (CAT) levels in gallstone disease: Cholecystectomy patients with high plasma levels of CAT have significantly lower number of analgesic … []
Malaysian Journal of Medicine and Health Sciences Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring []
nature environment pollution technology Toxic Trace Metals and Pathological Changes in Organs of Rats Fed with Extract of Polluted Grasses []
World Neurosurg Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ¡­ [33689849]
Biomed Res Int Mechanisms of Trx2/ASK1-Mediated Mitochondrial Injury in Pemphigus Vulgaris [33763469]
Nutrients Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and?¡­ [34064937]
Molecular Medicine Reports Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats [34278476]
J Pers Med Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis [34945751]
In Vivo Plasma Concentration of the Lipid Peroxidation (LP) Biomarker 4-Ηydroxynonenal (4-HNE) in Benign and Cancer Patients [Pubmed:35241533]
Biochimica et Biophysica Acta-Molecular Basis of Disease α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat diet [Pubmed:35569738]
Antioxidants Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory … [Pubmed:35204215]
留言咨询