皮质酮(Cort)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Corticosterone (Cort)

Corticosrone

  • 皮质酮(Cort)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 皮质酮(Cort)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 皮质酮(Cort)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA540Ge.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测皮质酮(Cort),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的皮质酮(Cort)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 79-97 88
EDTA plasma(n=5) 79-89 82
heparin plasma(n=5) 98-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的皮质酮(Cort),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中皮质酮(Cort)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 87-102% 93-101% 78-91% 81-105%
EDTA plasma(n=5) 99-105% 78-92% 80-89% 79-95%
heparin plasma(n=5) 84-92% 89-98% 88-98% 80-101%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将皮质酮(Cort)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA540Ge11 皮质酮(Cort)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA540Ge21 皮质酮(Cort)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA540Ge31 皮质酮(Cort)钥孔血蓝蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA540Ge01 皮质酮(Cort)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA540Ge71 皮质酮(Cort)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA540Ge81 皮质酮(Cort)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA540Ge22 皮质酮(Cort)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA540Ge72 皮质酮(Cort)单克隆抗体(生物素标记) WB; IHC; ICC.
CEA540Ge 皮质酮(Cort)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA540Ge 皮质酮(Cort)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA540Ge 皮质酮(Cort)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA540Ge11 皮质酮(Cort)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Respir Res. Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. [PubMed: 20403205]
Natural Product Sciences Effects of Gypenosides on Acute Stress in Mice [Papersearch: Source]
Neuropeptides.? Role of adrenomedullin in the cerebrospinal fluid-contacting nucleus in the modulation of immobilization stress [Pubmed:25911494]
EXPERIMENTAL &amp; CLINICAL CARDIOLOGY Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice [Com:Source]
Journal of Pharmacological Sciences Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized Rats [Pubmed:24366191]
Toxicology Letters Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats [Pubmed:24440345]
Stress. Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation [Pubmed:25238024]
Int J Clin Exp Med Diabetes alters the blood glucose response to ketamine in streptozotocin-diabetic rats [PubMed: 26379948]
Pakistan Veterinary Journal Trivalent Chromium Attenuated Corticosterone Secretion and Actions in Adrenocorticotropic Hormone-Stimulated Rats [In_Press: 15-058.Pdf]
BMC Ameliorating effects of gypenosides on chronic stress-induced anxiety disorders in mice [PubMed: 26370834]
Behav Brain Res Rats with differential self-grooming expression in the elevated plus-maze do not differ in anxiety-related behaviors [PubMed: 26142783]
Archives of Physiology and Biochemistry Improvement of oral contraceptive-induced glucose dysreCavia (Guinea pig )lation and dyslipidemia by valproic acid is independent of circulating corticosterone [Pubmed:26855320]
Archives of Physiology and Biochemistry Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder [Pubmed:26934364]
Neuroscience Letters Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression [Pubmed:26773866]
Neural Regeneration Research Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression. [Pubmed:27212922]
Environmental Science and Pollution Research Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway [Pubmed:27102619]
Eur Neuropsychopharmacol Apelin-13 exerts antidepressant-like and recognition memory improving activities in stressed rats [Pubmed:26853763]
experimental brain research Dopamine D2-like receptors modulate freezing response, but not the activation of HPA axis, during the expression of conditioned fear. [pubmed:27766352]
brain research bulletin Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. [pubmed:28161195]
Neurochemical Research  Effects of (-)-Sesamin on Chronic Stress-Induced Anxiety Disorders in Mice [pubmed:27995494]
Journal of Molecular Histology Role of serotonin in the intestinal mucosal epithelium barrier in weaning mice undergoing stress-induced diarrhea [pubmed:29260435]
Psychosomatic Medicine The Role of Psychological Stress on Heart Autophagy in Mice With Heart Failure [pubmed:28691995]
Scientific Reports COMMUNICATIONS Environmental perturbation of the circadian clock during pregnancy leads to transgenerational mood disorder-like behaviors in mice [pubmed:28974783]
BMC Gastroenterology Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice [pubmed:28633646]
biorxiv Anxiety-like but not despair-like behaviors are further aggravated by chronic mild stress in the early stages of APPswe/PS1dE9 transgenic mice [doi.org/10.1101/202283]
Frontiers in Molecular Neuroscience Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis. [pubmed:28959186]
Biochemical Pharmacology Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female rats [Pubmed:30028991]
Toxicological Sciences Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci]
Nutrition Multigenerational effects of dietary macronutrients intake on the metabolic phenotype of male Wistar rats [10.1016:j.nut.2018.06.008]
Behavioural Brain Research Depressive-and anxiety-like phenotypes in young adult APPSwe/PS1dE9 transgenic mice with insensitivity to chronic mild stress [Pubmed:30012417]
Stress Blockade of AT1 type receptors for angiotensin II prevents cardiac microvascular fibrosis induced by chronic stress in Sprague–Dawley rats [Pubmed:29676198]
Inflammation Differential Effects of Brain Death on Rat Microcirculation and Intestinal Inflammation: Female Male [Pubmed:29737476]
Respiratory physiology & neurobiology Curcumin attenuates chronic intermittent hypoxia-induced brain injuries by inhibiting AQP4 and p38 MAPK pathway [Pubmed:29758366]
Nature and Science of Sleep Chronic treatment with dexamethasone alters clock gene expression and melatonin synthesis in rat pineal gland at night [Pubmed:30046256]
Journal of Pharmacy and Pharmacology Tannins from fruits attenuates GABA antagonist‐induced anxiety‐like behaviour modulation of neurotransmitters [Pubmed: 30198561]
Bulletin of Experimental Biology and Medicine Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in … [Pubmed: 30353332]
Brain, Behavior, and Immunity HMGB1-Mediated Differential Response on Hippocampal Neurotransmitter Disorder and Neuroinflammation in Adolescent Male and Female Mice following Cold Exposure [Pubmed: 30476565]
Heliyon Establishment and evaluation of a novel mouse model of peri/postmenopausal depression [Doi: 10.1016/j.heliyon.2019.e01195]
Scientific Reports Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition [Pubmed: 31015500]
Food & Function Antidepressant-like effects of dietary gardenia blue pigment derived from genipin and tyrosine [Doi: 10.1039/C9FO00480G]
Neuroscience Letters Synaptic structure and alterations in the hippocampus in neonatal rats exposed to lipopolysaccharide [Pubmed: 31288048]
FASEB Journal Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice [Pubmed: 31431066]
Cells MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model [Pubmed: 31540304]
endocrine journal Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose … [Pubmed: 32249244]
INTERNATIONAL IMMUNOPHARMACOLOGY GABA-mediated activated microglia induce neuroinflammation in the hippocampus of mice following cold exposure through the NLRP3 inflammasome and … [Pubmed: 33166810]
neurochemical research Anxiolytic and Anti‑depressive Like Effects of Translocator Protein (18 kDa) Ligand YL‑IPA08 in a Rat Model of Postpartum Depression [Pubmed: 32383026]
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
博士论文 Neurobiological determinants of depressive-like symptoms in rodents []
Behavioral Ecology and Sociobiology  Exposure to eagle owl feces induces anti-predator behavior, physiology, and hypothalamic gene responses in a subterranean rodent, the plateau zokor (Eospalax … []
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