细胞色素C(CYCS)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Cytochrome C (CYCS)

Cyt-C; HCS; CYC; CytC

  • 细胞色素C(CYCS)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞色素C(CYCS)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞色素C(CYCS)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA594Mi.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒对检测细胞色素C(CYCS)具有较高的灵敏度。
经检测人、小鼠、大鼠之间有100%的交叉反映。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将细胞色素C(CYCS)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的细胞色素C(CYCS)与连接于固相载体上的抗体结合,然后加入生物素化的细胞色素C(CYCS)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的细胞色素C(CYCS)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA594Mi01 细胞色素C(CYCS)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA594Mi01 细胞色素C(CYCS)多克隆抗体 WB; IHC; ICC; IP.
SEA594Mi 细胞色素C(CYCS)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA594Mi01 细胞色素C(CYCS)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
J Ethnopharmacol Danhong injection protects cardiomyocytes against hypoxia/reoxygenation- and H2O2-induced injury by inhibiting mitochondrial permeability transition pore opening [PubMed: 26320687]
Cell Stress Chaperones Gypenosides alleviate myocardial ischemia-reperfusion injury via attenuation of oxidative stress and preservation of mitochondrial function in rat heart [Pubmed:26800973]
Molecules Clematichinenoside (AR) Attenuates Hypoxia/Reoxygenation-Induced H9c2 Cardiomyocyte Apoptosis via a Mitochondria-Mediated Signaling Pathway [Pubmed:27248986]
International Journal of Biological Macromolecules A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in rats [pubmed:28709897]
West Indian Medical Journal Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia. []
PeerJ. Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway [Pubmed: 30723634]
Biological trace element research High Concentrations of Boric Acid Trigger Concentration-Dependent Oxidative Stress, Apoptotic Pathways and Morphological Alterations in DU-145 Human Prostate … [Pubmed: 31066018]
Biological Trace Element Research The Role of Oxidative Stress, Renal Inflammation, and Apoptosis in Post Ischemic Reperfusion Injury of Kidney Tissue: the Protective Effect of Dose-Dependent Boric … []
Cell Stress Chaperones Betaine suppresses cell proliferation by increasing oxidative stress–mediated apoptosis and inflammation in DU-145 human prostate cancer cell line [Pubmed: 31368044]
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