内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Endothelin 1 (EDN1)

ET1; PPET1; Preproendothelin-1; Big endothelin-1

  • 内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA482Cp.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测内皮素1(EDN1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的内皮素1(EDN1)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 79-91 81
heparin plasma(n=5) 79-92 83

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的内皮素1(EDN1),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中内皮素1(EDN1)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 88-96% 82-97% 78-91%
EDTA plasma(n=5) 97-105% 81-93% 92-101% 89-96%
heparin plasma(n=5) 97-105% 81-90% 99-105% 80-104%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将内皮素1(EDN1)单克隆抗体包被微孔板,制成固相载 体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Capra hircus; Caprine (Goat,山羊) 应用(仅供研究使用,不用于临床诊断!)
CEA482Cp 内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Heart and Circulatory Physiology B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness [APS: source]
PLOS ONE CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source]
36 Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1 [PubMed: PMC3695508]
Clinics (Sao Paulo). Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic mice [Pubmed: 24212843]
Life Sci. Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model [Pubmed:25476829]
Transpl Int. One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys [Pubmed:24977654]
J Endocrinol Invest.? The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders [Pubmed:25245337]
Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880]
Journal of Surgical Research Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioning [Sciencedirect:S0022480414004909]
Pharmaceutical Biology The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafil [PubMed: 26023989]
A Periodical of the Hungarian Academy of Sciences The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patients [PubMed: 26690031]
Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880]
Human & Experimental Toxicology An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injury [PubMed: 26078283]
Artery Research Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome [science:article]
Transplant  proc Cardiovascular Risk Factors and Markers of Atherosclerosis in Stable Kidney TransplantRecipients. [pubmed:27496444]
Biomed Journal Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X. [pubmed:27884380]
International Journal of Advanced Research in Biological Sciences Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactose [pdfcopy:42552]
Journal of Drug Metabolisn& Toxicology Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose [587e5b6508aed3826af45e2d.pdf]
Polish Journal of Veterinary Sciences Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edema [pubmed:27760029]
Artery Research Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome [article:S1872-9312(16)30085-0]
Journal of Molecular Biomarkers J & Diagnosis Biochemical and Molecular Markers of Congenital and Senile Cataractous Lenses [DOI: 10.4172/2155-9929.1000318]
A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats. [AN=123819331]
Journal of Functional Foods Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation [10.1016/j.jff.2017.05.033]
Radiation Research Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute … [Pubmed:29809108]
Scandinavian Journal of Clinical and Laboratory Investigation Isolated kidney perfusion: the influence of pulsatile flow [Pubmed:29301417]
Endokrynologia Polska Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension … [Pubmed: 30359461]
Journal of Pharmacological Sciences Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome [Pubmed: 30342783]
Molecular Nutrition & Food Research Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight … [Pubmed: 30570214]
Journal of Cosmetic Dermatology Whitening effects of cosmetic formulation in the vascular component of skin pigmentation [Pubmed: 31074159]
Eurasian Journal of Clinical Sciences APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan []
Journal of Functional Foods Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease []
留言咨询