ELISA Kit for Endothelin 1 (EDN1)

ET1; PPET1; Preproendothelin-1; Big endothelin-1

  • 内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA482Cp.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证





样本 回收率范围(%) 平均回收率(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 79-91 81
heparin plasma(n=5) 79-92 83


精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%



样本 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 88-96% 82-97% 78-91%
EDTA plasma(n=5) 97-105% 81-93% 92-101% 89-96%
heparin plasma(n=5) 97-105% 81-90% 99-105% 80-104%




1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。


本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将内皮素1(EDN1)单克隆抗体包被微孔板,制成固相载 体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。


编号 适用物种:Capra hircus; Caprine (Goat,山羊) 应用(仅供研究使用,不用于临床诊断!)
CEA482Cp 内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.


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