瘦素(LEP)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Leptin (LEP)

OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

  • 瘦素(LEP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 瘦素(LEP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 瘦素(LEP)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA084Eq.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测瘦素(LEP),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的瘦素(LEP)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 88-105 95
EDTA plasma(n=5) 86-95 92
heparin plasma(n=5) 78-101 95

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的瘦素(LEP),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中瘦素(LEP)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 84-104% 91-102% 86-94% 91-98%
EDTA plasma(n=5) 93-102% 80-89% 88-101% 96-105%
heparin plasma(n=5) 82-99% 90-101% 79-97% 92-102%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将瘦素(LEP)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的瘦素(LEP)与连接于固相载体上的抗体结合,然后加入生物素化的瘦素(LEP)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的瘦素(LEP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Equus caballus; Equine (Horse,马) 应用(仅供研究使用,不用于临床诊断!)
RPA084Eq02 瘦素(LEP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA084Eq01 瘦素(LEP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA084Eq02 瘦素(LEP)多克隆抗体 WB; IHC; ICC; IP.
PAA084Eq01 瘦素(LEP)多克隆抗体 WB; IHC; ICC; IP.
MAA084Eq21 瘦素(LEP)单克隆抗体 WB; IHC; ICC; IP.
SEA084Eq 瘦素(LEP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
European Journal of Pharmacology Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet [PubMed: 20869961]
Indian Journal of Pharmacology Effect of Argyreia speciosa root extract on cafeteria diet-induced obesity in rats [Ijp: source]
Journal of endocrinology Testosterone protects from metabolic syndrome-associated prostate inflammation: an experimental study in rabbit [PubMed: 22010203]
PLoS One. Multi-Functional Roles of Chitosan as a Potential Protective Agent against Obesity [PubMed: 23342013]
Molecular and Cellular Endocrinology Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs [ScienceDirect: S0303720712004194]
Journal of Animal and Feed Sciences Ovarian, metabolic and endocrine indexes in dairy cows with different body condition scores [Ifzz: Source]
British Journal of Nutrition n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs [Pubmed: 23947577]
Int J Mol Sci. Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. [Pubmed: 24736781]
Endocr J. CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes [Pubmed:25168658]
Animal Physiology EFFECT OF LEPTIN GENE ON SERUM LEPTIN CONCENTRATION AND BEEF QUALITY IN CZECH FLECKVIEH BULLS [Mendelu:Source]
Evid Based Complement Alternat Med. Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats [Pubmed:Pmc4363645]
general endocrinology Oxytocin reverse ovariectomy-induced Osteoper and body fat gain [Pubmed:24506069]
Respiratory Physiology &amp; Neurobiology Maternal high-fat diet feeding during pregnancy and lactation augments lung inflammation and remodeling in the offspring [Pubmed:25500158]
Sci Rep Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis [PubMed: 26634430]
Evid Based Complement Alternat Med Tinospora crispa [PubMed: 25821506]
Ankara Effects of breeder age and early energy restriction on fattening performance, some meat quality traits and plasma leptin concentration of broilers [Dergiler: 11]
MEDICINA VETERIN Comparative measurements of the morphometric pattern and energy profile of Crioula mares in the final third of gestation, with different body condition scores [Scielo: S0102-09352015000300707]
Pancreatic Disorders & Therapy Pancreatic Exocrine Insufficiency Affects Not Only Digestibility of Nutrients and Growth, but Also Body Composition and Endocrinological Parameters – Study on Piglets Used As a Model for Children [Journals: 2165-7092-%20S5-00]
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers [Pubmed:27051212]
Animal Anterior pituitary influence on adipokine expression and secretion by porcine adipocytes. [Pubmed:27074715]
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens [journalprintahead:87]
Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens [view:87]
Obes Surg. Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. [Pubmed:27185175]
Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines [science:S0065128116301234]
J Biomed Mater Res A Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice [Pubmed:26850371]
Clin Epigenetics. The bromodomain inhibitor N-methyl pyrrolidone reduced fat accumulation in an ovariectomized rat model [Pubmed:27110299]
International Journal of Molecular Sciences Oral Supplementation with a Special Additive of Retinyl Palmitate and Alpha Tocopherol Reduces Growth Retardation in Young Pancreatic Duct Ligated Pigs Used as a Model for Children Suffering from Exocrine Pancreatic Insufficiency [pubmed:27690005]
Journal of Animal Science Changes in small intestinal morphometry and electrogenic nutrient transport in young pigs with pancreatic exocrine insufficiency [publications:jas]
International Journal of Endocrinology The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages [pubmed:27990162]
journal of animal science The effect of GnRH vaccination on performance, carcass, and meat quality and hormonal regulation in boars, barrows, and gilts. [pubmed:27482668]
journal of animal science Diet-induced modifications to milk composition have long-term effects on offspring growth in rabbits. [pubmed:28380600]
Evidence-Based Complementary and Alternative Medicine Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. [pubmed:28246535]
Asian-Australasian Journal of Animal Sciences Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits [pubmed:27004817]
Stem Cell Research & Therapy Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizingradiation. [pubmed:27530249]
Obesity Surgery Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in ObeseGöttingen Minipig. Effects on Glucose Metabolism. [pubmed:27185175]
Meat Sci Estimation of the intramuscular fat content of m. longissimus thoracis in crossbred beef cattle based on live animal measurements. [pubmed:27940421]
THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY Correlation of Leptin, Insulin and Glucose During Late Gestation and Early Lactation in Murrah Buffaloes [7086]
Journal of Animal and Plant Sciences Effects Of Maca (Lepıdıum Meyenıı Walp) Powder On Serum Indıces And Metabolıc Responses In Racehorses [57cf073008ae83b37462323a.pdf]
Biological Trace Element Research Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet [article:10.1007]
Revista Brasileira de Ciência Avícola Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken [10.159:1806-9061-2016-0285]
Applied Animal Behaviour Science Evaluating measures of exploratory behaviour in sows around farrowing and during lactation− A pilot study [10.1016/j.jacl.2017.03.007]
Proceedings of the National Academy of Sciences of the United States of America (PNAS) cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis [Pubmed:29784793]
Oxidative Medicine and Cellular Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … [Pubmed:29849871]
Frontiers in Endocrinology Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in … [Pubmed:29755407]
Journal of Agricultural and Food Chemistry Metabolomics for the effect of biotin and nicotinamide on transition dairy cows [Pubmed:29758980]
Frontiers in Physiology Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 [Pubmed:29593555]
Bioscience Reports Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats [Pubmed:29784871]
Neurochemical Research The leptin, dopamine and serotonin receptors in hypothalamic POMC-neurons of normal and obese rodents [Pubmed:29397535]
PLoS One Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens [Pubmed:29373588]
Biochemical and Biophysical Research Communications Chronic cold exposure results in subcutaneous adipose tissue browning and altered global metabolism in Qinghai-Tibetan plateau pika (Ochotona curzoniae) [Pubmed:29626477]
Alcohol and Alcoholism Leptin Expression and Gene Methylation Patterns in Alcohol-Dependent Patients with Ethyltoxic Cirrhosis—Normalization After Liver Transplantation and Implications … [Pubmed:29912265]
Iranian Red Crescent Medical Journal  The Effect of Aerobic Training on Tumor Growth, Adiponectin, Leptin and Ghrelin in Mice with Breast Cancer [ircmj-20-03-13305.pdf]
Pakistan Journal of Zoology Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. [:]
Oncotarget Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … [Pubmed:29484120]
УСПЕХИ ГЕРОНТОЛОГИИ ВЛИЯНИЕ МЕТФОРМИНА НА МЕТАБОЛИЧЕСКИЕ ПОКАЗАТЕЛИ И ГИПОТАЛАМИЧЕСКИЕ СИГНАЛЬНЫЕ СИСТЕМЫ У КРЫС С ОЖИРЕНИЕМ … [AG_2018-31-01.pdf]
留言咨询